Institut for Biomedicin

Annemarie Brüel

Loss of Bone Strength is Dependent on Skeletal Site in Disuse Osteoporosis in Rats

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Loss of Bone Strength is Dependent on Skeletal Site in Disuse Osteoporosis in Rats. / Thomsen, Jesper Skovhus; Christensen, Lisbeth Lydiksen; Vegger, Jens Bay; Nyengaard, Jens Randel; Brüel, Annemarie.

I: Calcified Tissue International, Bind 90, Nr. 4, 2012, s. 294-306.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Thomsen, Jesper Skovhus ; Christensen, Lisbeth Lydiksen ; Vegger, Jens Bay ; Nyengaard, Jens Randel ; Brüel, Annemarie. / Loss of Bone Strength is Dependent on Skeletal Site in Disuse Osteoporosis in Rats. I: Calcified Tissue International. 2012 ; Bind 90, Nr. 4. s. 294-306.

Bibtex

@article{a5fa615b154f4dd985e49f39642f9d8d,
title = "Loss of Bone Strength is Dependent on Skeletal Site in Disuse Osteoporosis in Rats",
abstract = "Intramuscular injection with botulinum toxin A (BTX) leads to a transient paralysis of the muscles, resulting in a rapid loss of muscle mass and function as well as rapid bone loss (disuse osteoporosis). The purpose of this study was to investigate the temporal development and the site specificity of BTX-induced immobilization on bone strength at five skeletal sites. Three-month-old rats (n = 108) were randomized into nine groups: one served as baseline, while four were injected with BTX and four with saline in the right hind-limb musculature. Animals were killed after 1, 2, 3, or 4 weeks. BTX-induced a significant loss of rectus femoris muscle mass (-61%) and muscle cell cross-sectional area (-59%) as well as bone strength at the femoral neck (-31%), femoral diaphysis (-6%), distal femoral metaphysis (-17%), proximal tibial metaphysis (-31%), and tibial diaphysis (-13%) after 4 weeks. Muscle atrophy occurred in parallel with the bone loss at the femoral neck and proximal tibia, whereas it occurred earlier than the bone loss at the other skeletal sites. At the proximal tibial metaphysis BTX significantly decreased BV/TV (-10%), trabecular thickness (-13%), and bone formation (MS/BS -25%, BFR/BS -50%) and increased osteoclast covered surfaces (+97%) after 4 weeks. In conclusion, BTX-induced a time-dependent loss of bone strength. Moreover, the loss of bone strength differed significantly at the five tested skeletal sites.",
author = "Thomsen, {Jesper Skovhus} and Christensen, {Lisbeth Lydiksen} and Vegger, {Jens Bay} and Nyengaard, {Jens Randel} and Annemarie Br{\"u}el",
year = "2012",
doi = "10.1007/s00223-012-9576-7",
language = "English",
volume = "90",
pages = "294--306",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York LLC",
number = "4",

}

RIS

TY - JOUR

T1 - Loss of Bone Strength is Dependent on Skeletal Site in Disuse Osteoporosis in Rats

AU - Thomsen, Jesper Skovhus

AU - Christensen, Lisbeth Lydiksen

AU - Vegger, Jens Bay

AU - Nyengaard, Jens Randel

AU - Brüel, Annemarie

PY - 2012

Y1 - 2012

N2 - Intramuscular injection with botulinum toxin A (BTX) leads to a transient paralysis of the muscles, resulting in a rapid loss of muscle mass and function as well as rapid bone loss (disuse osteoporosis). The purpose of this study was to investigate the temporal development and the site specificity of BTX-induced immobilization on bone strength at five skeletal sites. Three-month-old rats (n = 108) were randomized into nine groups: one served as baseline, while four were injected with BTX and four with saline in the right hind-limb musculature. Animals were killed after 1, 2, 3, or 4 weeks. BTX-induced a significant loss of rectus femoris muscle mass (-61%) and muscle cell cross-sectional area (-59%) as well as bone strength at the femoral neck (-31%), femoral diaphysis (-6%), distal femoral metaphysis (-17%), proximal tibial metaphysis (-31%), and tibial diaphysis (-13%) after 4 weeks. Muscle atrophy occurred in parallel with the bone loss at the femoral neck and proximal tibia, whereas it occurred earlier than the bone loss at the other skeletal sites. At the proximal tibial metaphysis BTX significantly decreased BV/TV (-10%), trabecular thickness (-13%), and bone formation (MS/BS -25%, BFR/BS -50%) and increased osteoclast covered surfaces (+97%) after 4 weeks. In conclusion, BTX-induced a time-dependent loss of bone strength. Moreover, the loss of bone strength differed significantly at the five tested skeletal sites.

AB - Intramuscular injection with botulinum toxin A (BTX) leads to a transient paralysis of the muscles, resulting in a rapid loss of muscle mass and function as well as rapid bone loss (disuse osteoporosis). The purpose of this study was to investigate the temporal development and the site specificity of BTX-induced immobilization on bone strength at five skeletal sites. Three-month-old rats (n = 108) were randomized into nine groups: one served as baseline, while four were injected with BTX and four with saline in the right hind-limb musculature. Animals were killed after 1, 2, 3, or 4 weeks. BTX-induced a significant loss of rectus femoris muscle mass (-61%) and muscle cell cross-sectional area (-59%) as well as bone strength at the femoral neck (-31%), femoral diaphysis (-6%), distal femoral metaphysis (-17%), proximal tibial metaphysis (-31%), and tibial diaphysis (-13%) after 4 weeks. Muscle atrophy occurred in parallel with the bone loss at the femoral neck and proximal tibia, whereas it occurred earlier than the bone loss at the other skeletal sites. At the proximal tibial metaphysis BTX significantly decreased BV/TV (-10%), trabecular thickness (-13%), and bone formation (MS/BS -25%, BFR/BS -50%) and increased osteoclast covered surfaces (+97%) after 4 weeks. In conclusion, BTX-induced a time-dependent loss of bone strength. Moreover, the loss of bone strength differed significantly at the five tested skeletal sites.

U2 - 10.1007/s00223-012-9576-7

DO - 10.1007/s00223-012-9576-7

M3 - Journal article

C2 - 22354132

VL - 90

SP - 294

EP - 306

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 4

ER -