Institut for Biomedicin

Annemarie Brüel

Inhibition of cross-links in collagen is associated with reduced stiffness of the aorta in young rats

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Inhibition of cross-links in collagen is associated with reduced stiffness of the aorta in young rats. / Brüel, A; Ortoft, G; Oxlund, H.

I: Atherosclerosis, Bind 140, Nr. 1, 1998, s. 135-45.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Brüel, A, Ortoft, G & Oxlund, H 1998, 'Inhibition of cross-links in collagen is associated with reduced stiffness of the aorta in young rats', Atherosclerosis, bind 140, nr. 1, s. 135-45.

APA

CBE

MLA

Vancouver

Author

Brüel, A ; Ortoft, G ; Oxlund, H. / Inhibition of cross-links in collagen is associated with reduced stiffness of the aorta in young rats. I: Atherosclerosis. 1998 ; Bind 140, Nr. 1. s. 135-45.

Bibtex

@article{abe56480d8f811de9e3b000ea68e967b,
title = "Inhibition of cross-links in collagen is associated with reduced stiffness of the aorta in young rats",
abstract = "Collagen and elastin fibres are of major importance in providing the aorta with tensile strength and elasticity. The presence of cross-links in collagen and elastin is essential for the mechanical stability of collagen and elastin fibres. beta-aminopropionitrile (BAPN) reduces the formation of cross-links by inhibiting the enzyme lysyloxidase. Young rats were injected with BAPN to inhibit the formation of cross-links, and the changes in the biomechanical and biochemical properties of the thoracic aorta were studied. The biomechanical analyses of aortic samples from BAPN-treated rats showed a significantly increased diameter (1.64 +/-0.02 mm), a significantly reduced maximum load (1.08+/-0.08 N), and a significantly reduced maximum stiffness (3.34+/-0.10 N) compared with controls (1.57+/-0.02 mm, 1.55+/-0.04 N and 4.49 +/-0.14 N, respectively). No changes in the concentrations of collagen and elastin were found. The content of pyridinoline, a mature collagen cross-link, was significantly decreased by 49% in the BAPN-treated group compared with controls. No changes in the concentration of desmosine + isodesmosine, the major cross-links of elastin. were found. The present study shows that cross-links are essential in providing mechanical stability of the aorta. Even a partial inhibition of the cross-linking processes results in a destabilisation of the aortic wall with increased diameter and reduced strength and stiffness.",
keywords = "Aging, Amino Acids, Animals, Aorta, Collagen, Desmosine, Elasticity, Elastin, Female, Rats, Rats, Wistar, Structure-Activity Relationship",
author = "A Br{\"u}el and G Ortoft and H Oxlund",
year = "1998",
language = "English",
volume = "140",
pages = "135--45",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Inhibition of cross-links in collagen is associated with reduced stiffness of the aorta in young rats

AU - Brüel, A

AU - Ortoft, G

AU - Oxlund, H

PY - 1998

Y1 - 1998

N2 - Collagen and elastin fibres are of major importance in providing the aorta with tensile strength and elasticity. The presence of cross-links in collagen and elastin is essential for the mechanical stability of collagen and elastin fibres. beta-aminopropionitrile (BAPN) reduces the formation of cross-links by inhibiting the enzyme lysyloxidase. Young rats were injected with BAPN to inhibit the formation of cross-links, and the changes in the biomechanical and biochemical properties of the thoracic aorta were studied. The biomechanical analyses of aortic samples from BAPN-treated rats showed a significantly increased diameter (1.64 +/-0.02 mm), a significantly reduced maximum load (1.08+/-0.08 N), and a significantly reduced maximum stiffness (3.34+/-0.10 N) compared with controls (1.57+/-0.02 mm, 1.55+/-0.04 N and 4.49 +/-0.14 N, respectively). No changes in the concentrations of collagen and elastin were found. The content of pyridinoline, a mature collagen cross-link, was significantly decreased by 49% in the BAPN-treated group compared with controls. No changes in the concentration of desmosine + isodesmosine, the major cross-links of elastin. were found. The present study shows that cross-links are essential in providing mechanical stability of the aorta. Even a partial inhibition of the cross-linking processes results in a destabilisation of the aortic wall with increased diameter and reduced strength and stiffness.

AB - Collagen and elastin fibres are of major importance in providing the aorta with tensile strength and elasticity. The presence of cross-links in collagen and elastin is essential for the mechanical stability of collagen and elastin fibres. beta-aminopropionitrile (BAPN) reduces the formation of cross-links by inhibiting the enzyme lysyloxidase. Young rats were injected with BAPN to inhibit the formation of cross-links, and the changes in the biomechanical and biochemical properties of the thoracic aorta were studied. The biomechanical analyses of aortic samples from BAPN-treated rats showed a significantly increased diameter (1.64 +/-0.02 mm), a significantly reduced maximum load (1.08+/-0.08 N), and a significantly reduced maximum stiffness (3.34+/-0.10 N) compared with controls (1.57+/-0.02 mm, 1.55+/-0.04 N and 4.49 +/-0.14 N, respectively). No changes in the concentrations of collagen and elastin were found. The content of pyridinoline, a mature collagen cross-link, was significantly decreased by 49% in the BAPN-treated group compared with controls. No changes in the concentration of desmosine + isodesmosine, the major cross-links of elastin. were found. The present study shows that cross-links are essential in providing mechanical stability of the aorta. Even a partial inhibition of the cross-linking processes results in a destabilisation of the aortic wall with increased diameter and reduced strength and stiffness.

KW - Aging

KW - Amino Acids

KW - Animals

KW - Aorta

KW - Collagen

KW - Desmosine

KW - Elasticity

KW - Elastin

KW - Female

KW - Rats

KW - Rats, Wistar

KW - Structure-Activity Relationship

M3 - Journal article

C2 - 9733224

VL - 140

SP - 135

EP - 145

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 1

ER -