Institut for Biomedicin

Annemarie Brüel

Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength. / Henriksen, Kim; Flores, Carmen; Thomsen, Jesper Skovhus; Brüel, Annemarie; Thudium, Christian S; Neutzsky-Wulff, Anita V; Langenbach, Geerling E J; Sims, Natalie; Askmyr, Maria; Martin, Thomas J; Everts, Vincent; Karsdal, Morten A; Richter, Johan.

I: P L o S One, Bind 6, Nr. 11, 2011, s. e27482.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Henriksen, K, Flores, C, Thomsen, JS, Brüel, A, Thudium, CS, Neutzsky-Wulff, AV, Langenbach, GEJ, Sims, N, Askmyr, M, Martin, TJ, Everts, V, Karsdal, MA & Richter, J 2011, 'Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength', P L o S One, bind 6, nr. 11, s. e27482. https://doi.org/10.1371/journal.pone.0027482

APA

Henriksen, K., Flores, C., Thomsen, J. S., Brüel, A., Thudium, C. S., Neutzsky-Wulff, A. V., Langenbach, G. E. J., Sims, N., Askmyr, M., Martin, T. J., Everts, V., Karsdal, M. A., & Richter, J. (2011). Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength. P L o S One, 6(11), e27482. https://doi.org/10.1371/journal.pone.0027482

CBE

Henriksen K, Flores C, Thomsen JS, Brüel A, Thudium CS, Neutzsky-Wulff AV, Langenbach GEJ, Sims N, Askmyr M, Martin TJ, Everts V, Karsdal MA, Richter J. 2011. Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength. P L o S One. 6(11):e27482. https://doi.org/10.1371/journal.pone.0027482

MLA

Vancouver

Author

Henriksen, Kim ; Flores, Carmen ; Thomsen, Jesper Skovhus ; Brüel, Annemarie ; Thudium, Christian S ; Neutzsky-Wulff, Anita V ; Langenbach, Geerling E J ; Sims, Natalie ; Askmyr, Maria ; Martin, Thomas J ; Everts, Vincent ; Karsdal, Morten A ; Richter, Johan. / Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength. I: P L o S One. 2011 ; Bind 6, Nr. 11. s. e27482.

Bibtex

@article{eeaaea41a9dd4083a3ca6a02671d7666,
title = "Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength",
abstract = "Osteopetrosis caused by defective acid secretion by the osteoclast, is characterized by defective bone resorption, increased osteoclast numbers, while bone formation is normal or increased. In contrast the bones are of poor quality, despite this uncoupling of formation from resorption.To shed light on the effect of uncoupling in adult mice with respect to bone strength, we transplanted irradiated three-month old normal mice with hematopoietic stem cells from control or oc/oc mice, which have defective acid secretion, and followed them for 12 to 28 weeks.Engraftment levels were assessed by flow cytometry of peripheral blood. Serum samples were collected every six weeks for measurement of bone turnover markers. At termination bones were collected for µCT and mechanical testing. An engraftment level of 98% was obtained. From week 6 until termination bone resorption was significantly reduced, while the osteoclast number was increased when comparing oc/oc to controls. Bone formation was elevated at week 6, normalized at week 12, and reduced onwards. µCT and mechanical analyses of femurs and vertebrae showed increased bone volume and bone strength of cortical and trabecular bone.In conclusion, these data show that attenuation of acid secretion in adult mice leads to uncoupling and improves bone strength.",
author = "Kim Henriksen and Carmen Flores and Thomsen, {Jesper Skovhus} and Annemarie Br{\"u}el and Thudium, {Christian S} and Neutzsky-Wulff, {Anita V} and Langenbach, {Geerling E J} and Natalie Sims and Maria Askmyr and Martin, {Thomas J} and Vincent Everts and Karsdal, {Morten A} and Johan Richter",
year = "2011",
doi = "10.1371/journal.pone.0027482",
language = "English",
volume = "6",
pages = "e27482",
journal = "P L o S One",
issn = "1932-6203",
publisher = "public library of science",
number = "11",

}

RIS

TY - JOUR

T1 - Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength

AU - Henriksen, Kim

AU - Flores, Carmen

AU - Thomsen, Jesper Skovhus

AU - Brüel, Annemarie

AU - Thudium, Christian S

AU - Neutzsky-Wulff, Anita V

AU - Langenbach, Geerling E J

AU - Sims, Natalie

AU - Askmyr, Maria

AU - Martin, Thomas J

AU - Everts, Vincent

AU - Karsdal, Morten A

AU - Richter, Johan

PY - 2011

Y1 - 2011

N2 - Osteopetrosis caused by defective acid secretion by the osteoclast, is characterized by defective bone resorption, increased osteoclast numbers, while bone formation is normal or increased. In contrast the bones are of poor quality, despite this uncoupling of formation from resorption.To shed light on the effect of uncoupling in adult mice with respect to bone strength, we transplanted irradiated three-month old normal mice with hematopoietic stem cells from control or oc/oc mice, which have defective acid secretion, and followed them for 12 to 28 weeks.Engraftment levels were assessed by flow cytometry of peripheral blood. Serum samples were collected every six weeks for measurement of bone turnover markers. At termination bones were collected for µCT and mechanical testing. An engraftment level of 98% was obtained. From week 6 until termination bone resorption was significantly reduced, while the osteoclast number was increased when comparing oc/oc to controls. Bone formation was elevated at week 6, normalized at week 12, and reduced onwards. µCT and mechanical analyses of femurs and vertebrae showed increased bone volume and bone strength of cortical and trabecular bone.In conclusion, these data show that attenuation of acid secretion in adult mice leads to uncoupling and improves bone strength.

AB - Osteopetrosis caused by defective acid secretion by the osteoclast, is characterized by defective bone resorption, increased osteoclast numbers, while bone formation is normal or increased. In contrast the bones are of poor quality, despite this uncoupling of formation from resorption.To shed light on the effect of uncoupling in adult mice with respect to bone strength, we transplanted irradiated three-month old normal mice with hematopoietic stem cells from control or oc/oc mice, which have defective acid secretion, and followed them for 12 to 28 weeks.Engraftment levels were assessed by flow cytometry of peripheral blood. Serum samples were collected every six weeks for measurement of bone turnover markers. At termination bones were collected for µCT and mechanical testing. An engraftment level of 98% was obtained. From week 6 until termination bone resorption was significantly reduced, while the osteoclast number was increased when comparing oc/oc to controls. Bone formation was elevated at week 6, normalized at week 12, and reduced onwards. µCT and mechanical analyses of femurs and vertebrae showed increased bone volume and bone strength of cortical and trabecular bone.In conclusion, these data show that attenuation of acid secretion in adult mice leads to uncoupling and improves bone strength.

U2 - 10.1371/journal.pone.0027482

DO - 10.1371/journal.pone.0027482

M3 - Journal article

C2 - 22087326

VL - 6

SP - e27482

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 11

ER -