Institut for Biomedicin

Annemarie Brüel

Calcified cartilage islands in rat cortical bone

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Calcified cartilage islands in rat cortical bone. / Bach-Gansmo, Fiona Linnea; Irvine, Sarah Catherine; Brüel, Annemarie; Thomsen, Jesper Skovhus; Birkedal, Henrik.

I: Calcified Tissue International, Bind 92, Nr. 4, 04.2013, s. 330-8.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Bach-Gansmo, FL, Irvine, SC, Brüel, A, Thomsen, JS & Birkedal, H 2013, 'Calcified cartilage islands in rat cortical bone', Calcified Tissue International, bind 92, nr. 4, s. 330-8. https://doi.org/10.1007/s00223-012-9682-6

APA

Bach-Gansmo, F. L., Irvine, S. C., Brüel, A., Thomsen, J. S., & Birkedal, H. (2013). Calcified cartilage islands in rat cortical bone. Calcified Tissue International, 92(4), 330-8. https://doi.org/10.1007/s00223-012-9682-6

CBE

MLA

Bach-Gansmo, Fiona Linnea o.a.. "Calcified cartilage islands in rat cortical bone". Calcified Tissue International. 2013, 92(4). 330-8. https://doi.org/10.1007/s00223-012-9682-6

Vancouver

Bach-Gansmo FL, Irvine SC, Brüel A, Thomsen JS, Birkedal H. Calcified cartilage islands in rat cortical bone. Calcified Tissue International. 2013 apr;92(4):330-8. https://doi.org/10.1007/s00223-012-9682-6

Author

Bach-Gansmo, Fiona Linnea ; Irvine, Sarah Catherine ; Brüel, Annemarie ; Thomsen, Jesper Skovhus ; Birkedal, Henrik. / Calcified cartilage islands in rat cortical bone. I: Calcified Tissue International. 2013 ; Bind 92, Nr. 4. s. 330-8.

Bibtex

@article{94cafd4d8d4640bbbc0a404b6044d1c3,
title = "Calcified cartilage islands in rat cortical bone",
abstract = "Rats display little to no haversian remodeling of cortical bone. This fact, combined with the endochondral formation of cortical bone, means that rat femoral cortical bone contains highly mineralized cartilage islands in a central band of mid-femoral cross sections. We demonstrate that these islands have a significantly higher degree of mineralization than the surrounding bone, using quantitative backscattered electron imaging. The cartilaginous nature of the islands was verified by immunostaining for collagen type II. Toluidine blue staining of longitudinal sections and three-dimensional synchrotron radiation X-ray tomographic microscopy confirmed that the islands are elongated along the femoral long axis. Nanoindentation revealed significantly higher values of both reduced modulus and hardness in the islands compared to the surrounding bone, reflecting a higher degree of mineralization. The calcified cartilage islands were distributed in a central zone of the bone, from the growth plates through the mid-femoral bone. The presence of these cartilage islands and their possible effect on mechanical properties could be an additional reason why haversian remodeling is observed in higher-order species.",
author = "Bach-Gansmo, {Fiona Linnea} and Irvine, {Sarah Catherine} and Annemarie Br{\"u}el and Thomsen, {Jesper Skovhus} and Henrik Birkedal",
year = "2013",
month = apr,
doi = "10.1007/s00223-012-9682-6",
language = "English",
volume = "92",
pages = "330--8",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York LLC",
number = "4",

}

RIS

TY - JOUR

T1 - Calcified cartilage islands in rat cortical bone

AU - Bach-Gansmo, Fiona Linnea

AU - Irvine, Sarah Catherine

AU - Brüel, Annemarie

AU - Thomsen, Jesper Skovhus

AU - Birkedal, Henrik

PY - 2013/4

Y1 - 2013/4

N2 - Rats display little to no haversian remodeling of cortical bone. This fact, combined with the endochondral formation of cortical bone, means that rat femoral cortical bone contains highly mineralized cartilage islands in a central band of mid-femoral cross sections. We demonstrate that these islands have a significantly higher degree of mineralization than the surrounding bone, using quantitative backscattered electron imaging. The cartilaginous nature of the islands was verified by immunostaining for collagen type II. Toluidine blue staining of longitudinal sections and three-dimensional synchrotron radiation X-ray tomographic microscopy confirmed that the islands are elongated along the femoral long axis. Nanoindentation revealed significantly higher values of both reduced modulus and hardness in the islands compared to the surrounding bone, reflecting a higher degree of mineralization. The calcified cartilage islands were distributed in a central zone of the bone, from the growth plates through the mid-femoral bone. The presence of these cartilage islands and their possible effect on mechanical properties could be an additional reason why haversian remodeling is observed in higher-order species.

AB - Rats display little to no haversian remodeling of cortical bone. This fact, combined with the endochondral formation of cortical bone, means that rat femoral cortical bone contains highly mineralized cartilage islands in a central band of mid-femoral cross sections. We demonstrate that these islands have a significantly higher degree of mineralization than the surrounding bone, using quantitative backscattered electron imaging. The cartilaginous nature of the islands was verified by immunostaining for collagen type II. Toluidine blue staining of longitudinal sections and three-dimensional synchrotron radiation X-ray tomographic microscopy confirmed that the islands are elongated along the femoral long axis. Nanoindentation revealed significantly higher values of both reduced modulus and hardness in the islands compared to the surrounding bone, reflecting a higher degree of mineralization. The calcified cartilage islands were distributed in a central zone of the bone, from the growth plates through the mid-femoral bone. The presence of these cartilage islands and their possible effect on mechanical properties could be an additional reason why haversian remodeling is observed in higher-order species.

U2 - 10.1007/s00223-012-9682-6

DO - 10.1007/s00223-012-9682-6

M3 - Journal article

C2 - 23274728

VL - 92

SP - 330

EP - 338

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 4

ER -