Aarhus Universitets segl

Alexander Schmitz

B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes

Publikation: KonferencebidragKonferenceabstrakt til konferenceForskningpeer review

Standard

B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes. / Johnsen, Hans Erik; Schmitz, Alexander; Perez Andres, Martin et al.
2011. Abstract fra 13th International Myeloma Workshop, Paris, Frankrig.

Publikation: KonferencebidragKonferenceabstrakt til konferenceForskningpeer review

Harvard

Johnsen, HE, Schmitz, A, Perez Andres, M, Johansen, P, Bøgsted, M, Nyegaard, M, Bukh, A, Fogd, K, Orfao, A & Dybkær, K 2011, 'B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes', 13th International Myeloma Workshop, Paris, Frankrig, 03/05/2011 - 06/05/2011.

APA

Johnsen, H. E., Schmitz, A., Perez Andres, M., Johansen, P., Bøgsted, M., Nyegaard, M., Bukh, A., Fogd, K., Orfao, A., & Dybkær, K. (2011). B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes. Abstract fra 13th International Myeloma Workshop, Paris, Frankrig.

CBE

Johnsen HE, Schmitz A, Perez Andres M, Johansen P, Bøgsted M, Nyegaard M, Bukh A, Fogd K, Orfao A, Dybkær K. 2011. B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes. Abstract fra 13th International Myeloma Workshop, Paris, Frankrig.

MLA

Johnsen, Hans Erik et al. B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes. 13th International Myeloma Workshop, 03 maj 2011, Paris, Frankrig, Konferenceabstrakt til konference, 2011.

Vancouver

Johnsen HE, Schmitz A, Perez Andres M, Johansen P, Bøgsted M, Nyegaard M et al.. B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes. 2011. Abstract fra 13th International Myeloma Workshop, Paris, Frankrig.

Author

Johnsen, Hans Erik ; Schmitz, Alexander ; Perez Andres, Martin et al. / B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes. Abstract fra 13th International Myeloma Workshop, Paris, Frankrig.

Bibtex

@conference{eca21f7226114cf6b43c99a916d6b39a,
title = "B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes",
abstract = "In order to improve insights into the B-cell biology and thereby B-cell myelomagenesis we have established a MSCNET standard for multiparametric flow cytometry (MFC) and cell sorting (FACS) for subsequent genetic analysis. The material analysed was fresh tonsils, blood and bone marrow. The method included homogenization, isolation of mononuclear cells, MFC and FACS sorting using multicolour fluorescence single tube panels.of antibodies against surface molecules as CD10/20/27/38/45, supplemented with tissue related antibodies. Isolated B-cell subpopulations were evaluated by morphological inspection and single gene expression analysis (qRT-PCR) for transcription factors as well as global gene expression profiling (GEP; GeneChip Human Exon 1.0 ST Array). For example for tonsils, based on the immunophenotypic presentation (includingCD3/44/CXCR4 in the panel), B-cell subsets were identified and sorted, na{\"i}ve, centroblast, centrocyte, memory, and plasmablasts. The identity of the tonsillar subpopulations was verified using qRT-PCR and exon microarray GEP based on the used discriminative phenotypic markers as well as transcriptions factors BACH2, BCL6, PAX5, IRF4, P27, PRDM1 and XBP1. Globally, the B-cell subpopulations identified have distinct gene expression profiles reflecting their functions but also revealing genes with subpopulation specific exon splicing. In conclusion a combination of surface markers expressed antigens and gene expression analysis of B cell subsets confirm a strong methodology to be used in myelomagenesis and evaluated as for prognostic information.",
author = "Johnsen, {Hans Erik} and Alexander Schmitz and {Perez Andres}, Martin and P Johansen and Martin B{\o}gsted and Mette Nyegaard and Anne Bukh and Kirsten Fogd and Alberto Orfao and Karen Dybk{\ae}r",
year = "2011",
month = may,
day = "6",
language = "English",
note = "13th International Myeloma Workshop ; Conference date: 03-05-2011 Through 06-05-2011",

}

RIS

TY - ABST

T1 - B-cell subpopulations from normal human secondary lymphoid tissues with specific gene expression profiles and phenotypes

AU - Johnsen, Hans Erik

AU - Schmitz, Alexander

AU - Perez Andres, Martin

AU - Johansen, P

AU - Bøgsted, Martin

AU - Nyegaard, Mette

AU - Bukh, Anne

AU - Fogd, Kirsten

AU - Orfao, Alberto

AU - Dybkær, Karen

PY - 2011/5/6

Y1 - 2011/5/6

N2 - In order to improve insights into the B-cell biology and thereby B-cell myelomagenesis we have established a MSCNET standard for multiparametric flow cytometry (MFC) and cell sorting (FACS) for subsequent genetic analysis. The material analysed was fresh tonsils, blood and bone marrow. The method included homogenization, isolation of mononuclear cells, MFC and FACS sorting using multicolour fluorescence single tube panels.of antibodies against surface molecules as CD10/20/27/38/45, supplemented with tissue related antibodies. Isolated B-cell subpopulations were evaluated by morphological inspection and single gene expression analysis (qRT-PCR) for transcription factors as well as global gene expression profiling (GEP; GeneChip Human Exon 1.0 ST Array). For example for tonsils, based on the immunophenotypic presentation (includingCD3/44/CXCR4 in the panel), B-cell subsets were identified and sorted, naïve, centroblast, centrocyte, memory, and plasmablasts. The identity of the tonsillar subpopulations was verified using qRT-PCR and exon microarray GEP based on the used discriminative phenotypic markers as well as transcriptions factors BACH2, BCL6, PAX5, IRF4, P27, PRDM1 and XBP1. Globally, the B-cell subpopulations identified have distinct gene expression profiles reflecting their functions but also revealing genes with subpopulation specific exon splicing. In conclusion a combination of surface markers expressed antigens and gene expression analysis of B cell subsets confirm a strong methodology to be used in myelomagenesis and evaluated as for prognostic information.

AB - In order to improve insights into the B-cell biology and thereby B-cell myelomagenesis we have established a MSCNET standard for multiparametric flow cytometry (MFC) and cell sorting (FACS) for subsequent genetic analysis. The material analysed was fresh tonsils, blood and bone marrow. The method included homogenization, isolation of mononuclear cells, MFC and FACS sorting using multicolour fluorescence single tube panels.of antibodies against surface molecules as CD10/20/27/38/45, supplemented with tissue related antibodies. Isolated B-cell subpopulations were evaluated by morphological inspection and single gene expression analysis (qRT-PCR) for transcription factors as well as global gene expression profiling (GEP; GeneChip Human Exon 1.0 ST Array). For example for tonsils, based on the immunophenotypic presentation (includingCD3/44/CXCR4 in the panel), B-cell subsets were identified and sorted, naïve, centroblast, centrocyte, memory, and plasmablasts. The identity of the tonsillar subpopulations was verified using qRT-PCR and exon microarray GEP based on the used discriminative phenotypic markers as well as transcriptions factors BACH2, BCL6, PAX5, IRF4, P27, PRDM1 and XBP1. Globally, the B-cell subpopulations identified have distinct gene expression profiles reflecting their functions but also revealing genes with subpopulation specific exon splicing. In conclusion a combination of surface markers expressed antigens and gene expression analysis of B cell subsets confirm a strong methodology to be used in myelomagenesis and evaluated as for prognostic information.

M3 - Conference abstract for conference

T2 - 13th International Myeloma Workshop

Y2 - 3 May 2011 through 6 May 2011

ER -