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Albert Johannes Buitenhuis

Transcriptional profiling of the bovine hepatic response to experimentally induced E. coli mastitis

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Transcriptional profiling of the bovine hepatic response to experimentally induced E. coli mastitis. / Jørgensen, Hanne Birgitte Hede; Buitenhuis, Bart; Røntved, Christine Maria; Jiang, Li; Ingvartsen, Klaus Lønne; Sørensen, Peter.

I: Physiological Genomics, Bind 44, Nr. 11, 01.06.2012, s. 595-606.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Jørgensen, Hanne Birgitte Hede ; Buitenhuis, Bart ; Røntved, Christine Maria ; Jiang, Li ; Ingvartsen, Klaus Lønne ; Sørensen, Peter. / Transcriptional profiling of the bovine hepatic response to experimentally induced E. coli mastitis. I: Physiological Genomics. 2012 ; Bind 44, Nr. 11. s. 595-606.

Bibtex

@article{8a1fb18904274b63b234f0b19b534b82,
title = "Transcriptional profiling of the bovine hepatic response to experimentally induced E. coli mastitis",
abstract = "The mammalian liver works to keep the body in a state of homeostasis and plays an important role in systemic acute phase response to infections. In this study we investigated the bovine hepatic acute phase response at the gene transcription level in dairy cows with experimentally E. coli-induced mastitis. At time = 0, each of 16 periparturient dairy cows received 20-40 CFU of live E. coli in one front quarter of the udder. A time series of liver biopsies was collected at -144, 12, 24 and 192 hours relative to time of inoculation. Changes in transcription levels in response to E. coli inoculation were analyzed using the Bovine Genome Array and tested significant for 408 transcripts over the time series (adjusted p0.05; abs(fold-change)>2). After 2-D clustering, transcripts represented three distinct transcription profiles: 1) regulation of gene transcription and apoptosis, 2) responses to cellular stress invoked by reactive metabolites, and 3) metabolism and turnover of proteins. The results showed that the liver went through a period of perturbations to its normal homeostatic condition during the first 24 hours following the E. coli-induced intra-mammary inflammation. In previous studies, bacterial lipopolysaccaride, LPS, was used for intra-mammary stimulation to mimic E. coli infection. Comparing responses to LPS and E. coli, induced biochemical processes were similar but not identical (94% and 85% similarity between corresponding samples at early and late acute phase, respectively), but their kinetics were not. A notable difference concerned transcription of factors associated with oxidative stress in E. coli-induced liver responses",
keywords = "Gene expression, acute phase response, LPS, gram negative bacteria",
author = "J{\o}rgensen, {Hanne Birgitte Hede} and Bart Buitenhuis and R{\o}ntved, {Christine Maria} and Li Jiang and Ingvartsen, {Klaus L{\o}nne} and Peter S{\o}rensen",
year = "2012",
month = jun,
day = "1",
doi = "10.1152/physiolgenomics.00084.2011",
language = "English",
volume = "44",
pages = "595--606",
journal = "Physiological Genomics",
issn = "1094-8341",
publisher = "American Physiological Society",
number = "11",

}

RIS

TY - JOUR

T1 - Transcriptional profiling of the bovine hepatic response to experimentally induced E. coli mastitis

AU - Jørgensen, Hanne Birgitte Hede

AU - Buitenhuis, Bart

AU - Røntved, Christine Maria

AU - Jiang, Li

AU - Ingvartsen, Klaus Lønne

AU - Sørensen, Peter

PY - 2012/6/1

Y1 - 2012/6/1

N2 - The mammalian liver works to keep the body in a state of homeostasis and plays an important role in systemic acute phase response to infections. In this study we investigated the bovine hepatic acute phase response at the gene transcription level in dairy cows with experimentally E. coli-induced mastitis. At time = 0, each of 16 periparturient dairy cows received 20-40 CFU of live E. coli in one front quarter of the udder. A time series of liver biopsies was collected at -144, 12, 24 and 192 hours relative to time of inoculation. Changes in transcription levels in response to E. coli inoculation were analyzed using the Bovine Genome Array and tested significant for 408 transcripts over the time series (adjusted p0.05; abs(fold-change)>2). After 2-D clustering, transcripts represented three distinct transcription profiles: 1) regulation of gene transcription and apoptosis, 2) responses to cellular stress invoked by reactive metabolites, and 3) metabolism and turnover of proteins. The results showed that the liver went through a period of perturbations to its normal homeostatic condition during the first 24 hours following the E. coli-induced intra-mammary inflammation. In previous studies, bacterial lipopolysaccaride, LPS, was used for intra-mammary stimulation to mimic E. coli infection. Comparing responses to LPS and E. coli, induced biochemical processes were similar but not identical (94% and 85% similarity between corresponding samples at early and late acute phase, respectively), but their kinetics were not. A notable difference concerned transcription of factors associated with oxidative stress in E. coli-induced liver responses

AB - The mammalian liver works to keep the body in a state of homeostasis and plays an important role in systemic acute phase response to infections. In this study we investigated the bovine hepatic acute phase response at the gene transcription level in dairy cows with experimentally E. coli-induced mastitis. At time = 0, each of 16 periparturient dairy cows received 20-40 CFU of live E. coli in one front quarter of the udder. A time series of liver biopsies was collected at -144, 12, 24 and 192 hours relative to time of inoculation. Changes in transcription levels in response to E. coli inoculation were analyzed using the Bovine Genome Array and tested significant for 408 transcripts over the time series (adjusted p0.05; abs(fold-change)>2). After 2-D clustering, transcripts represented three distinct transcription profiles: 1) regulation of gene transcription and apoptosis, 2) responses to cellular stress invoked by reactive metabolites, and 3) metabolism and turnover of proteins. The results showed that the liver went through a period of perturbations to its normal homeostatic condition during the first 24 hours following the E. coli-induced intra-mammary inflammation. In previous studies, bacterial lipopolysaccaride, LPS, was used for intra-mammary stimulation to mimic E. coli infection. Comparing responses to LPS and E. coli, induced biochemical processes were similar but not identical (94% and 85% similarity between corresponding samples at early and late acute phase, respectively), but their kinetics were not. A notable difference concerned transcription of factors associated with oxidative stress in E. coli-induced liver responses

KW - Gene expression

KW - acute phase response

KW - LPS

KW - gram negative bacteria

U2 - 10.1152/physiolgenomics.00084.2011

DO - 10.1152/physiolgenomics.00084.2011

M3 - Journal article

C2 - 22496490

VL - 44

SP - 595

EP - 606

JO - Physiological Genomics

JF - Physiological Genomics

SN - 1094-8341

IS - 11

ER -