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Albert Johannes Buitenhuis

RNA sequencing based analysis of the spleen transcriptome following the infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations

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  • Edin Hamzic
  • ,
  • Rikke Brødsgaard Kjærup
  • Núria Mach , INRA, UMR1313 GABI, Jouy-en-Josas, France, Frankrig
  • Guilietta Minozzi, Parco Tecnologico Padano, PTP, University of Milan, Italien
  • Francesco Strozzi, Parco Tecnologico Padano, Italien
  • Valentina Gualdi, Parco Tecnologico Padano, Italien
  • John L Williams, Parco Tecnologico Padana, Lodi, University of Adelaide, Italien
  • Jun Chen, Cobb-Vantress Ins., USA
  • Eva Wattrang, National Veterinary Institute, Uppsala, Sverige
  • Albert Johannes Buitenhuis
  • Helle Risdahl Juul-Madsen, Danmark
  • Tina Sørensen Dalgaard
BackgroundAvian infectious bronchitis (IB) is an acute and highly contagious disease of the upper-respiratory tract caused by infectious bronchitis virus (IBV). Understanding the molecular mechanisms involved in the immune response to IBV infection is a crucial element for further improvements in strategies to control IB. To this end, two chicken lines, selected for high and low serum concentration of mannose-binding lectin (MBL), a soluble pattern recognition receptor, were studied. In total, 32 animals from each line (designated L10H for high and L10L for low MBL serum concentration) were used. Sixteen birds from each line were infected with IBV on day 1 and birds were euthanized at 1 week and 3 weeks post infection, 8 uninfected controls and 8 infected birds from each line at each occasion. RNA sequencing was performed on spleen samples from all 64 birds used in the experiment. Differential gene expression analysis was performed for four comparisons: L10L line versus L10H line for control animals at week 1 and week 3, respectively and L10L line versus L10H line for infected animals at week 1 and week 3, respectively. Functional analysis based on the differentially expressed genes was performed using Gene Ontology (GO) Immune System Process terms specific for Gallus gallus.ResultsComparing uninfected L10H and L10L birds, we identified 1698 and 1934 differentially expressed (DE) genes at week 1 and week 3, respectively. For the IBV infected birds 1698 and 1934 DE genes were identified between two lines at week 1 and week 3, respectively with an FDR adjusted p-value <0.05. The two most enriched GO terms comparing uninfected birds from the two lines at week 1 and week 3 were “Lymphocyte activation involved in immune response” (GO:0002285) and “Somatic recombination of immunoglobulin genes involved in immune response” (GO:0002204), respectively. When comparing IBV infected birds between of the two lines, the most enriched GO terms were “Alpha-beta T cell activation” (GO:0046631) and “Positive regulation of leukocyte activation” (GO:0002696) at week 1 and week 3, respectively.ConclusionsHealthy animals from the two lines showed significant differences in expression profiles for subsets of both adaptive and innate immunity related genes. Whereas, comparison of the IBV infected birds from the two MBL lines showed differences in expression of immunity related genes involved in T cell activation and proliferation. The observed transcriptome differences between the two lines indicate that selection for MBL had a much wider effect. Future research will focus on identifying signatures of selection in order to further understand molecular pathways being responsible for differences between the two lines.
TidsskriftBMC Genomics
StatusUdgivet - 27 jan. 2016

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