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Albert Johannes Buitenhuis

Factors influencing milk osteopontin concentration based on measurements from Danish Holstein cows

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Factors influencing milk osteopontin concentration based on measurements from Danish Holstein cows. / Christensen, Brian; Zachariae, Elias D; Poulsen, Nina A; Buitenhuis, Albert J; Larsen, Lotte B; Sørensen, Esben S.

I: Journal of Dairy Research, Bind 88, Nr. 1, 02.2021, s. 89 - 94.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{cc3da1af971b4d6a83fb7e8e2db8b873,
title = "Factors influencing milk osteopontin concentration based on measurements from Danish Holstein cows",
abstract = "Our objective was to determine the content of the bioactive protein osteopontin (OPN) in bovine milk and identify factors influencing its concentration. OPN is expressed in many tissues and body fluids, with by far the highest concentrations in milk. OPN plays a role in immunological and developmental processes and it has been associated with several milk production traits and lactation persistency in cows. In the present study, we report the development of an enzyme linked immunosorbent assay (ELISA) for measurement of OPN in bovine milk. The method was used to determine the concentration of OPN in milk from 661 individual Danish Holstein cows. The median OPN level was determined to 21.9 mg/l with a pronounced level of individual variation ranging from 0.4 mg/l to 67.8 mg/l. Breeding for increased OPN in cow's milk is of significant interest, however, the heritability of OPN in milk was found to be relatively low, with an estimated value of 0.19 in the current dataset. The variation explained by the herd was also found to be low suggesting that OPN levels are not affected by farm management or feeding. Interestingly, the concentration of OPN was found to increase with days in milk and to decrease with parity.",
keywords = "Bioactive milk protein, ELISA, Osteopontin, heritability",
author = "Brian Christensen and Zachariae, {Elias D} and Poulsen, {Nina A} and Buitenhuis, {Albert J} and Larsen, {Lotte B} and S{\o}rensen, {Esben S}",
year = "2021",
month = feb,
doi = "10.1017/S0022029921000054",
language = "English",
volume = "88",
pages = "89 -- 94",
journal = "Journal of Dairy Research",
issn = "0022-0299",
publisher = "Cambridge University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Factors influencing milk osteopontin concentration based on measurements from Danish Holstein cows

AU - Christensen, Brian

AU - Zachariae, Elias D

AU - Poulsen, Nina A

AU - Buitenhuis, Albert J

AU - Larsen, Lotte B

AU - Sørensen, Esben S

PY - 2021/2

Y1 - 2021/2

N2 - Our objective was to determine the content of the bioactive protein osteopontin (OPN) in bovine milk and identify factors influencing its concentration. OPN is expressed in many tissues and body fluids, with by far the highest concentrations in milk. OPN plays a role in immunological and developmental processes and it has been associated with several milk production traits and lactation persistency in cows. In the present study, we report the development of an enzyme linked immunosorbent assay (ELISA) for measurement of OPN in bovine milk. The method was used to determine the concentration of OPN in milk from 661 individual Danish Holstein cows. The median OPN level was determined to 21.9 mg/l with a pronounced level of individual variation ranging from 0.4 mg/l to 67.8 mg/l. Breeding for increased OPN in cow's milk is of significant interest, however, the heritability of OPN in milk was found to be relatively low, with an estimated value of 0.19 in the current dataset. The variation explained by the herd was also found to be low suggesting that OPN levels are not affected by farm management or feeding. Interestingly, the concentration of OPN was found to increase with days in milk and to decrease with parity.

AB - Our objective was to determine the content of the bioactive protein osteopontin (OPN) in bovine milk and identify factors influencing its concentration. OPN is expressed in many tissues and body fluids, with by far the highest concentrations in milk. OPN plays a role in immunological and developmental processes and it has been associated with several milk production traits and lactation persistency in cows. In the present study, we report the development of an enzyme linked immunosorbent assay (ELISA) for measurement of OPN in bovine milk. The method was used to determine the concentration of OPN in milk from 661 individual Danish Holstein cows. The median OPN level was determined to 21.9 mg/l with a pronounced level of individual variation ranging from 0.4 mg/l to 67.8 mg/l. Breeding for increased OPN in cow's milk is of significant interest, however, the heritability of OPN in milk was found to be relatively low, with an estimated value of 0.19 in the current dataset. The variation explained by the herd was also found to be low suggesting that OPN levels are not affected by farm management or feeding. Interestingly, the concentration of OPN was found to increase with days in milk and to decrease with parity.

KW - Bioactive milk protein

KW - ELISA

KW - Osteopontin

KW - heritability

U2 - 10.1017/S0022029921000054

DO - 10.1017/S0022029921000054

M3 - Journal article

C2 - 33622420

VL - 88

SP - 89

EP - 94

JO - Journal of Dairy Research

JF - Journal of Dairy Research

SN - 0022-0299

IS - 1

ER -