Labelling of Cells Engaged in DNA Synthesis: Autoradiography and BrdU Staining

Publication: CommunicationInternet publication

  • Department of Medical Biochemistry
The cell cycle is divided in four phases: G1 phase, S phase (DNA-synthesis), G2 phase (together termed interphase) and M phase (mitosis). Cells that have ceased proliferation enter a state of quiescence called G0. M phase is itself composed of two tightly coupled processes: mitosis, in which the cell's chromosomes are divided between the two daughter cells, and cytokinesis, in which the cell's cytoplasm divides in half forming distinct new cells. It is often necessary to evaluate the proportion of DNA-replicating nuclei (S-phase cells) in a given population of cells. Classical methods for detection of cells in the S phase use incorporation of modified nucleosides, followed by direct visualisation of the DNA -replicating cells
Original languageEnglish
Publication year18 Oct 2010
Type Encyclopedia of Life Sciences
Source/PublisherJohn Wiley & Sons, Ltd
DOIs
StatePublished - 18 Oct 2010

    Keywords

  • cell cycle, DNA replication, cell proliferation, S phase, DNA synthesis, bromodeoxyuridine (BrdU), [3H]thymidine

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