Circulating miRNAs as biomarkers for oral squamous cell carcinoma recurrence in operated patients

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal article

  • Yan Yan
  • Xuan Wang, Capital Medical University, China
  • Morten Trillingsgaard Venø
  • Vivi Bakholdt, Odense University Hospital, DenmarkJens A. Sørensen, Odense University Hospital, UnknownAnnelise Krogdahl, Odense University Hospital, Zheng Sun, Capital Medical University, ChinaShan Gao, Suzhou Ribo Life Science Co., Ltd. , Beijing, China.,
  • Jørgen Kjems
MicroRNAs (miRNAs) are small regulatory non-coding RNAs for which altered expression in cancers can serve as potential biomarkers for diseases. We here investigated whether circulating miRNAs can serve as biomarkers for predicting post-operational recurrence of oral squamous cell carcinoma (OSCC) in patients. Plasma samples from 8 Danish OSCC patients were collected before, and one year after surgical operation, as well as from 3 Danish healthy controls and subjected to miRNA profiling by next generation sequencing. Disease recurrence did not occur in the 8 patients when the post-operative plasma samples were collected. Based on the sequencing data, three up-regulated miRNAs (miR-148a-3p, miR-26a-5p and miR-21-5p) and three down-regulated miRNAs (miR-375, miR-92b-3p and miR-486-5p) in the OSCC samples compared to healthy controls were selected for qRT-PCR validation in a Chinese cohort of 20 plasma samples collected before, and 9-12 months after surgical operation, and 18 healthy controls. Disease recurrence had occurred in 8 out of the 20 Chinese patients at the time their post-operative plasma samples were collected. The results of qRT-PCR showed that down-regulation of miR-486-5p, miR-375 and miR-92b-3p were highly associated with OSCC recurrence. This study indicates that the plasma miRNA profile is altered in OSCC during its progression and can be used to monitor the likelihood of OSCC recurrence in patients after surgery.
Original languageEnglish
JournalOncoTarget
Volume8
Issue number5
ISSN1949-2553
DOIs
StatePublished - 31 Jan 2017

    Research areas

  • NGS; OSCC; circulating miRNA; qRT–PCR; recurrence

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