Aerobic anoxygenic photosynthesis is commonly present within the genus Limnohabitans

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DOI

  • Vojtěch Kasalický, Biology Centre CAS, Institute of Hydrobiology, České Budějovice, Czech Republic vojtech.ves@post.cz.
  • ,
  • Yonghui Zeng
  • Kasia Piwosz, Institute of Microbiology CAS, Centre ALGATECH, Třeboň, Czech Republic.
  • ,
  • Karel Šimek, 1​Center Algatech, Institute of Microbiology CAS, Opatovický mlýn, 379 81 Třeboň, Czech Republic 2​Faculty of Science, University of South Bohemia, 370 05 České Budějovice, Czech Republic.
  • ,
  • Hana Kratochvilová, Biology Centre CAS, Institute of Hydrobiology, České Budějovice, Czech Republic.
  • ,
  • Michal Koblížek, 1​Center Algatech, Institute of Microbiology CAS, Opatovický mlýn, 379 81 Třeboň, Czech Republic 2​Faculty of Science, University of South Bohemia, 370 05 České Budějovice, Czech Republic.

The genus Limnohabitans (Comamonadaceae, Betaproteobacteria) is a common and a highly active component of freshwater bacterioplankton communities. So far, the genus has been considered to contain only heterotrophic species. In this study, we detected photosynthesis genes pufLM and bchY in 28 out of 46 strains from three Limnohabitans lineages. The obtained pufM sequences are very closely related to environmental pufM sequences detected in various freshwater habitats, indicating ubiquity and potential importance of photoheterotrophic Limnohabitans in nature.Additionally, we sequenced and analyzed the genomes of 5 potentially photoheterotrophic Limnohabitans strains to gain further insights into their phototrophic capacity. The structure of the photosynthesis gene cluster turned out to be highly conserved within the genus Limnohabitans and also among all potentially photosynthetic Betaproteobacteria The expression of photosynthetic complexes was detected in a culture of Limnohabitans planktonicus II-D5(T) using spectroscopic and pigment analyses. This was further verified by a novel combination of infra-red microscopy with fluorescent in situ hybridization.Importance The presented data document that the capacity to perform anoxygenic photosynthesis is common among the members of genus Limnohabitans, indicating that they may have a novel role in freshwater habitats.

Original languageEnglish
Article numbere02116-17
JournalApplied and Environmental Microbiology
Volume84
Issue1
Number of pages13
ISSN0099-2240
DOIs
Publication statusPublished - 2018

    Research areas

  • Bacteriochlorophyll, BchY, FISH, Freshwater Betaproteobacteria, IR microscopy, Limnohabitans, Photosynthetic bacteria, PufM

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