Niveispirillum lacus sp. nov., isolated from cyanobacterial aggregates in a eutrophic lake

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DOI

  • Haiyuan Cai, Chinese Academy of Sciences, China
  • Henglin Cui, Jiangsu University, China
  • Yonghui Zeng
  • Yanan Wang, Henan Academy of Sciences, China
  • Helong Jiang, Chinese Academy of Sciences, China

A bacterial strain, 1-14 T, was isolated from cyanobacterial aggregates in a eutrophic lake, Taihu Lake, China. Cells were observed to be slightly curved, rod-shaped, aerobic and Gram-stain-negative. Optimal growth occurred at pH 7.0 (range: 5.0-9.0), 28 °C (range: 20-32 °C) and 0% (w/v) NaCl (range: 0-1.0%) in R2A broth. No growth is observed at 37 °C. The cells were found to be positive for oxidase and catalase activities. The major respiratory quinone was ubiquinone Q-10. The major fatty acids (>10%) were identified as C 18:1ω6c/C 18:1ω7c, C1 6:0 3-OH and C 18:1 2-OH. The major polar lipids were found to consist of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine. Within the genus Niveispirillum, strain 1-14T was most closely related to Niveispirillum cyanobacteriorum TH16 T (98.3% 16S rRNA gene sequence similarity), followed by Niveispirillum irakense DSM 11586 T (97.8%) and Niveispirillum fermenti CC-LY736 T (97.0%). The genomic G+C content of strain 1-14 T was 62.2 mol% based on total genome calculations. Genes coding for light-harvesting complexes LHI and LHII, and a photosynthetic reaction centre were detected in the genome. Average nucleotide identities and digital DNA-DNA hybridizations for complete genomes ranged from 76.4 to 83.5 and from 21.5 to 27.4% between strain 1-14 T and strains within the genus Niveispirillum. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain 1-14 T represents a novel species within the genus Niveispirillum, for which the name Niveispirillum lacus sp. nov. is proposed. The type strain is 1-14 T (=CGMCC 1.12980 T=LMG 28363 T).

Original languageEnglish
Article number002526
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue2
Pages (from-to)507-512
Number of pages6
ISSN1466-5026
DOIs
Publication statusPublished - 2018

    Research areas

  • Cyanobacterial aggregates, Niveispirillum lacus sp. nov, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Phospholipids/chemistry, Phylogeny, Rhodospirillaceae/classification, Sequence Analysis, DNA, Ubiquinone/chemistry, Eutrophication, Lakes/microbiology, Base Composition, Bacterial Typing Techniques, China, Cyanobacteria/classification, Fatty Acids/chemistry, DNA, Bacterial/genetics

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