Comparison of autologous 111In-leukocytes, 18F-FDG, 11C-methionine, 11C-PK11195 and 68Ga-citrate for diagnostic nuclear imaging in a juvenile porcine haematogenous Staphylococcus aureus osteomyelitis model

Publication: ResearchConference abstract for conference

  • Ole L. Nielsen
    Ole L. NielsenDenmark
  • Pia Afzelius
    Pia AfzeliusDenmark
  • Dirk Bender
  • Henrik C. Schønheyder
    Henrik C. SchønheyderDenmark
  • Páll S. Leifsson
    Páll S. LeifssonDenmark
  • Karin M. Nielsen
    Karin M. NielsenDenmark
  • Jytte O. Larsen
    Jytte O. LarsenDenmark
  • Svend B. Jensen
    Svend B. JensenDenmark
  • Aage Kristian Olsen Alstrup
The aim of this study was to compare 111In-labeled leukocyte single-photon emission computed tomography (SPECT) and 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) to PET with tracers that potentially could improve detection of osteomyelitis. We chose 11C-methionine, 11C-PK11195 and 68Ga-citrate and validated their diagnostic utility in a porcine haematogenous osteomyelitis model. Four juvenile 14-15 weeks old female pigs were scanned seven days after intra-arterial inoculation in the right femoral artery with a porcine strain of Staphylococcus aureus using a sequential scan protocol with 18F-FDG, 68Ga-citrate, 11C-methionine, 11C-PK11195, 99mTc-Nanocoll and 111In-labelled autologous leukocytes. This was followed by necropsy of the pigs and gross pathology, histopathology and microbial examination. The pigs developed a total of five osteomyelitis lesions, five lesions characterized as abscesses/cellulitis, arthritis in three joints and five enlarged lymph nodes. None of the tracers accumulated in joints with arthritis. By comparing the 10 infectious lesions, 18F-FDG accumulated in nine, 111In-leukocytes in eight, 11C-methionine in six, 68Ga-citrate in four and 11C-PK11195 accumulated in only one lesion. Overall, 18F-FDG PET was superior to 111In-leukocyte SPECT in marking infectious and proliferative, i.e. hyperplastic, lesions. However, leukocyte SPECT was performed as early scans, approximately 6 h after injection of the leukocytes, to match the requirements of the 18 h long scan protocol. 11C-methionine and possibly 68Ga-citrate may be useful for diagnosis of soft issue lesions.
Original languageEnglish
Publication year21 Nov 2014
StatePublished - 21 Nov 2014
Event - København, Denmark

Seminar

SeminarSPECT and PET imaging in porcine inflammation and infection models
LocationKøbenhavns Universitet
CountryDenmark
CityKøbenhavn
Period21/11/201421/11/2014

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