Gender differences of oligomers and total adiponectin during puberty: a cross-sectional study of 859 Danish school children.

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  • Kristian Kjaer Andersen, Denmark
  • Jan Frystyk
  • Ole D Wolthers, Denmark
  • Carsten Heuck, Denmark
  • Allan Flyvbjerg, Denmark
  • The Department of Paediatrics
  • The Department of Endocrinology and Diabetes
  • Medical Research Laboratory
CONTEXT: Pubertal stages have been shown to influence total adiponectin (ADPN) levels. Furthermore, testosterone has been shown to alter the isomer distribution of ADPN. OBJECTIVE: The goal of this study was to investigate whether pubertal stages and testosterone levels influenced total serum ADPN levels and the distribution of ADPN isomers. DESIGN: This is a cross-sectional study. PATIENTS: The study included 859 children and adolescents (396 males) aged 6-20 yr. MAIN OUTCOME MEASURES: Total ADPN and ADPN isomers were measured using a validated in-house immunofluorometric assay. Fractioning of the ADPN into the three major molecular fractions was performed in representative subgroups of pre- and postpubertal males and females (n = 40, 10 in each group) using a validated fast protein liquid chromatography method. RESULTS: Total ADPN levels before puberty were 13.4 (11.1-15.9) mg/liter (median and interquartile range) and 14.7 (12.3-18.1) mg/liter (P = not significant), in males and females, respectively. After puberty, ADPN levels were significantly reduced in males, 9.7 (8.2-12.0) mg/liter but remained unchanged in females, 12.1 (9.7-15.3) mg/liter (P < 0.0001). Concomitantly, a reduction was seen in the ratio of high-molecular-weight (HMW) isomers to total ADPN (HMW ratio) when comparing prepubertal and postpubertal males. Also, postpubertal males had lower HMW ratios than corresponding females (P = 0.038). Finally, a negative correlation was seen between HMW ratio and testosterone (r = -0.430, P = 0.007). CONCLUSION: Serum total ADPN levels decrease through puberty in males. Also, a reduced HMW ratio is seen in males at the onset of puberty. We speculate that the suppression of HMW ADPN may be caused by testosterone.
Udgivelsesdato: 2007-May
Original languageEnglish
JournalJournal of Clinical Endocrinology and Metabolism
Volume92
Issue5
Pages (from-to)1857-62
Number of pages5
ISSN0021-972X
DOIs
Publication statusPublished - 2007

    Research areas

  • Adiponectin, Adolescent, Adult, Body Mass Index, Child, Chromatography, Gel, Chromatography, High Pressure Liquid, Cross-Sectional Studies, Denmark, Female, Fluoroimmunoassay, Humans, Immunoassay, Isomerism, Male, Puberty, Sex Characteristics, Testosterone

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