Extrachromosomal deoxyribonucleic acid in R factor-harboring Enterobacteriaceae.

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    JK Møller, DenmarkAL Bak, DenmarkC Christiansen, Denmark
  • Gunna Christiansen
  • A. Stenderup, Denmark
  • Department of Medical Microbiology and Immunology

Extrachromosomal deoxyribonucleic acid (DNA) from 24 different R factor-harboring Enterobacteriaceae was isolated and characterized by analytical ultracentrifugation and electron microscopy. The R factors represented 15 different patterns of transferable drug resistance found in enterobacteria from an enclosed geographic area. All of the strains contained extrachromosomal, circular DNA molecules within the range of 0.4 to 52 mum. More than one size class of circular DNA molecules was observed in the majority of the extrachromosomal DNA preparations. The buoyant density of the extrachromosomal DNA ranged from 1.700 to 1.720 g/cm3. The majority of the bacteria contained extrachromosomal DNAs of various densities. Three-fourths of the R factors were classified as fi+. The investigation illustrates the extensive variability in the physical characteristics of plasmid DNA from R factor-harboring strains.

Original languageEnglish
JournalJ Bacteriol
Pages (from-to)398-403
StatePublished - 1976

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