NMR-based metabonomic studies reveal changes in the biochemical profile of plasma and urine from pigs fed high fibre rye bread.

Research output: Research - peer-reviewJournal article

  • Hanne C. Bertram
    Hanne C. BertramDanish Institute of Agricultural SciencesDenmark
  • Knud E. Bach Knudsen
    Knud E. Bach KnudsenDanish Institute of Agricultural SciencesDenmark
  • Anja Serena
    Anja SerenaDanish Institute of Agricultural SciencesDenmark
  • Anders Malmendal
    Anders MalmendalDenmark
  • Niels Christian Nielsen
  • Xavier C. Fretté
    Xavier C. FrettéDanish Institute of Agricultural SciencesDenmark
  • Henrik J. Andersen
    Henrik J. AndersenDanish Institute of Agricultural SciencesDenmark
This study presents an NMR-based metabonomic approach to elucidate the overall endogenous biochemical effects of a wholegrain diet. Two diets with similar levels of dietary fibre and macronutrients, but with contrasting levels of wholegrain ingredients, were prepared from wholegrain rye (wholegrain diet (WGD)) and non-wholegrain wheat (non-wholegrain diet (NWD)) and fed to four pigs in a crossover design. Plasma samples were collected after 7 d on each diet, and 1H NMR spectra were acquired on these. Partial least squares regression discriminant analysis (PLS-DA) on spectra obtained for plasma samples revealed that the spectral region at 3.25 parts per million dominates the differentiation between the two diets, as the WGD is associated with higher spectral intensity in this region. Spiking experiments and LC-MS analyses of the plasma verified that this spectral difference could be ascribed to a significantly higher content of betaine in WGD plasma samples compared with NWD samples. In an identical study with the same diets, urine samples were collected, and 1H NMR spectra were acquired on these. PLS-DA on spectra obtained for urine samples revealed changes in the intensities of spectral regions, which could be ascribed to differences in the content of betaine and creatine/creatinine between the two diets, and LC-MS analyses verified a significantly lower content of creatinine in WGD urine samples compared with NWD urine samples. In conclusion, using an explorative approach, the present studies disclosed biochemical effects of a wholegrain diet on plasma betaine content and excretion of betaine and creatinine.
Original languageEnglish
JournalBritish J. Nutr.
Volume65
Pages (from-to)955-962
Number of pages8
StatePublished - 2006

    Research areas

  • NMR, betaine, creatinine, metabolomics, wholegrain

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