Profiling Osteogenic microRNAs For RNAi-Functionalization Of Scaffolds In Bone Tissue Engineering

Research output: Contribution to conferencePosterResearch

  • Chi-Chih (Clare) Chang
  • ,
  • Li Chen, Syddansk Universitet
  • ,
  • Morten Trillingsgaard Venø
  • ,
  • Moustapha Kassem, KMEB, Denmark
  • Jørgen Kjems
Bone remodeling and bone repair are natural processes occurring in the body throughout life. This metabolic process ensures that microfractures and microinjuries are repaired and thus maintaining healthy bones. However, when the damage is too large, such as from trauma injury or bone tumors, repair is limited and grafts are required to assist in bone repair. The use of allografts can cause immunological complications, whilst autografts subject the patient to two surgeries. Bone tissue engineering is a multidisciplinary field encompassing material science, medicine, chemistry and molecular biology aimed to produce a functional graft in vitro as an alternative to allografts and autografts. We explored the microRNAs (miRNAs) that aid in the bone formation process. MiRNAs are small non-coding RNAs of about 17-22 nucleotides in length that target the 3’UTR of mRNAs and represses their expression. MiRNAs have been found to facilitate many processes in the body including development, metabolism, and are implicated in many diseases. Many miRNAs have been identified with roles in osteogenesis, however a large systematic view at miRNA expression throughout osteogenesis that includes early, intermediate and late time points has yet to be done. We aimed to identify the expression profiles of miRNAs that as mesenchymal stem cells underwent osteogenesis with microRNA-sequencing. Most miRNAs were downregulated during osteogenesis and only few were upregulated. With the use of weighted gene correlated gene analysis we identified several expression profiles. Several miRNAs were validated in their osteogenic capabilities by overexpressing and knocking down the miRNAs, then assessing their ALP activities, Alizarin Red and ALP stainings and the expression of osteoblastic markers. In our screen we have identified both miRNAs that have been reported previously and many novel miRNAs with potent osteogenic capabilities. For tissue engineering applications, we then functionalized scaffolds with the miRNAs we identified and observed an increase in osteogenic capabilities in our 3D cultures. Our findings depicted the miRNA expression landscape as mesenchymal stem cells underwent osteogenic differentiation. We also highlight the potency of miRNAs as biological therapeutics in bone tissue engineering.
Original languageEnglish
Publication year2015
Publication statusPublished - 2015
EventNIH Symposium RNA Biology 2015 - NIH, Bethesda, United States
Duration: 11 Mar 2015 → …

Conference

ConferenceNIH Symposium RNA Biology 2015
LocationNIH, Bethesda
CountryUnited States
Period11/03/2015 → …

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