Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal article

Standard

Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures. / Manuguerra, Ilenia; Croce, Stefano; El-Sagheer, Afaf H.; Krissanaprasit, Abhichart; Brown, Tom; Gothelf, Kurt Vesterager; Manetto, Antonio.

In: Chemical Communications, Vol. 54, No. 36, 04.05.2018, p. 4529-4532.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal article

Harvard

Manuguerra, I, Croce, S, El-Sagheer, AH, Krissanaprasit, A, Brown, T, Gothelf, KV & Manetto, A 2018, 'Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures' Chemical Communications, vol 54, no. 36, pp. 4529-4532. DOI: 10.1039/C8CC00738A

APA

Manuguerra, I., Croce, S., El-Sagheer, A. H., Krissanaprasit, A., Brown, T., Gothelf, K. V., & Manetto, A. (2018). Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures. Chemical Communications, 54(36), 4529-4532. DOI: 10.1039/C8CC00738A

CBE

Manuguerra I, Croce S, El-Sagheer AH, Krissanaprasit A, Brown T, Gothelf KV, Manetto A. 2018. Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures. Chemical Communications. 54(36):4529-4532. Available from: 10.1039/C8CC00738A

MLA

Vancouver

Manuguerra I, Croce S, El-Sagheer AH, Krissanaprasit A, Brown T, Gothelf KV et al. Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures. Chemical Communications. 2018 May 4;54(36):4529-4532. Available from, DOI: 10.1039/C8CC00738A

Author

Manuguerra, Ilenia ; Croce, Stefano ; El-Sagheer, Afaf H. ; Krissanaprasit, Abhichart ; Brown, Tom ; Gothelf, Kurt Vesterager ; Manetto, Antonio. / Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures. In: Chemical Communications. 2018 ; Vol. 54, No. 36. pp. 4529-4532

Bibtex

@article{e7fcf4fd803d44fb8ec30aaf62a12960,
title = "Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures",
abstract = "Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.",
author = "Ilenia Manuguerra and Stefano Croce and El-Sagheer, {Afaf H.} and Abhichart Krissanaprasit and Tom Brown and Gothelf, {Kurt Vesterager} and Antonio Manetto",
year = "2018",
month = "5",
day = "4",
doi = "10.1039/C8CC00738A",
language = "English",
volume = "54",
pages = "4529--4532",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "ROYAL SOC CHEMISTRY",
number = "36",

}

RIS

TY - JOUR

T1 - Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

AU - Manuguerra,Ilenia

AU - Croce,Stefano

AU - El-Sagheer,Afaf H.

AU - Krissanaprasit,Abhichart

AU - Brown,Tom

AU - Gothelf,Kurt Vesterager

AU - Manetto,Antonio

PY - 2018/5/4

Y1 - 2018/5/4

N2 - Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

AB - Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

U2 - 10.1039/C8CC00738A

DO - 10.1039/C8CC00738A

M3 - Journal article

VL - 54

SP - 4529

EP - 4532

JO - Chemical Communications

T2 - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 36

ER -