Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

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Bjarkam, Carsten et al."Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal". Journal of Visualized Experiments. 2017. (122). Available: 10.3791/55511

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@article{1d0ea9275d1f471588f343df73f77f66,
title = "Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal",
keywords = "Journal Article",
author = "Carsten Bjarkam and Dariusz Orlowski and Laura Tvilling and Johannes Bech and Glud, {Andreas N} and Sørensen, {Jens Christian Hedemann}",
year = "2017",
month = "4",
doi = "10.3791/55511",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "Journal of Visualized Experiments",
number = "122",

}

RIS

TY - JOUR

T1 - Exposure of the Pig CNS for Histological Analysis

T2 - Journal of Visualized Experiments

AU - Bjarkam,Carsten

AU - Orlowski,Dariusz

AU - Tvilling,Laura

AU - Bech,Johannes

AU - Glud,Andreas N

AU - Sørensen,Jens Christian Hedemann

PY - 2017/4/13

Y1 - 2017/4/13

N2 - Pigs have become increasingly popular in large-animal translational neuroscience research as an economically and ethically feasible substitute to non-human primates. The large brain size of the pig allows the use of conventional clinical brain imagers and the direct use and testing of neurosurgical procedures and equipment from the human clinic. Further macroscopic and histological analysis, however, requires postmortem exposure of the pig central nervous system (CNS) and subsequent brain removal. This is not an easy task, as the pig CNS is encapsulated by a thick, bony skull and spinal column. The goal of this paper and instructional video is to describe how to expose and remove the postmortem pig brain and the pituitary gland in an intact state, suitable for subsequent macroscopic and histological analysis.

AB - Pigs have become increasingly popular in large-animal translational neuroscience research as an economically and ethically feasible substitute to non-human primates. The large brain size of the pig allows the use of conventional clinical brain imagers and the direct use and testing of neurosurgical procedures and equipment from the human clinic. Further macroscopic and histological analysis, however, requires postmortem exposure of the pig central nervous system (CNS) and subsequent brain removal. This is not an easy task, as the pig CNS is encapsulated by a thick, bony skull and spinal column. The goal of this paper and instructional video is to describe how to expose and remove the postmortem pig brain and the pituitary gland in an intact state, suitable for subsequent macroscopic and histological analysis.

KW - Journal Article

U2 - 10.3791/55511

DO - 10.3791/55511

M3 - Journal article

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 122

ER -