Docking of Antibodies into Cavities in DNA Origami

Research output: Research - peer-reviewJournal article

Standard

Docking of Antibodies into Cavities in DNA Origami. / Quyang , X; Stefano, Mattia De; Krissanaprasit, Abhichart; Kodal, Anne Louise Bank; Rosen, Christian Bech; Liu, T; Helmig, Sarah Wendelbo; Fan, C; Gothelf, Kurt Vesterager.

In: Angewandte Chemie (International Edition), Vol. 56, No. 46, 13.12.2017, p. 14423-14427.

Research output: Research - peer-reviewJournal article

Harvard

Quyang , X, Stefano, MD, Krissanaprasit, A, Kodal, ALB, Rosen, CB, Liu, T, Helmig, SW, Fan, C & Gothelf, KV 2017, 'Docking of Antibodies into Cavities in DNA Origami' Angewandte Chemie (International Edition), vol 56, no. 46, pp. 14423-14427. DOI: 10.1002/anie.201706765

APA

Quyang , X., Stefano, M. D., Krissanaprasit, A., Kodal, A. L. B., Rosen, C. B., Liu, T., ... Gothelf, K. V. (2017). Docking of Antibodies into Cavities in DNA Origami. Angewandte Chemie (International Edition), 56(46), 14423-14427. DOI: 10.1002/anie.201706765

CBE

Quyang X, Stefano MD, Krissanaprasit A, Kodal ALB, Rosen CB, Liu T, Helmig SW, Fan C, Gothelf KV. 2017. Docking of Antibodies into Cavities in DNA Origami. Angewandte Chemie (International Edition). 56(46):14423-14427. Available from: 10.1002/anie.201706765

MLA

Quyang , X et al."Docking of Antibodies into Cavities in DNA Origami". Angewandte Chemie (International Edition). 2017, 56(46). 14423-14427. Available: 10.1002/anie.201706765

Vancouver

Quyang X, Stefano MD, Krissanaprasit A, Kodal ALB, Rosen CB, Liu T et al. Docking of Antibodies into Cavities in DNA Origami. Angewandte Chemie (International Edition). 2017 Dec 13;56(46):14423-14427. Available from, DOI: 10.1002/anie.201706765

Author

Quyang , X ; Stefano, Mattia De ; Krissanaprasit, Abhichart ; Kodal, Anne Louise Bank ; Rosen, Christian Bech ; Liu, T ; Helmig, Sarah Wendelbo ; Fan, C ; Gothelf, Kurt Vesterager. / Docking of Antibodies into Cavities in DNA Origami. In: Angewandte Chemie (International Edition). 2017 ; Vol. 56, No. 46. pp. 14423-14427

Bibtex

@article{b89af7a5cf5f410a9976fe17ddb0c618,
title = "Docking of Antibodies into Cavities in DNA Origami",
abstract = "Immobilized antibodies are extensively employed for medical diagnostics such as in enzyme-linked immunosorbent assays. Despite their widespread use the ability to control the orientation on surfaces of immobilized antibodies is very limited. Herein, we report a method for covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNA origami structures. Two tris(NTA) modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysines. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) validated efficient antibody immobilization in the origami structures. The increased ability to control the orientation of antibodies in nanostructures and at surfaces has potential for directing the interactions of antibodies with targets and to provide more regular surface assemblies of antibodies.",
author = "X Quyang and Stefano, {Mattia De} and Abhichart Krissanaprasit and Kodal, {Anne Louise Bank} and Rosen, {Christian Bech} and T Liu and Helmig, {Sarah Wendelbo} and C Fan and Gothelf, {Kurt Vesterager}",
year = "2017",
month = "12",
doi = "10.1002/anie.201706765",
volume = "56",
pages = "14423--14427",
journal = "Angewandte Chemie (International Edition)",
issn = "1433-7851",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "46",

}

RIS

TY - JOUR

T1 - Docking of Antibodies into Cavities in DNA Origami

AU - Quyang ,X

AU - Stefano,Mattia De

AU - Krissanaprasit,Abhichart

AU - Kodal,Anne Louise Bank

AU - Rosen,Christian Bech

AU - Liu,T

AU - Helmig,Sarah Wendelbo

AU - Fan,C

AU - Gothelf,Kurt Vesterager

PY - 2017/12/13

Y1 - 2017/12/13

N2 - Immobilized antibodies are extensively employed for medical diagnostics such as in enzyme-linked immunosorbent assays. Despite their widespread use the ability to control the orientation on surfaces of immobilized antibodies is very limited. Herein, we report a method for covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNA origami structures. Two tris(NTA) modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysines. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) validated efficient antibody immobilization in the origami structures. The increased ability to control the orientation of antibodies in nanostructures and at surfaces has potential for directing the interactions of antibodies with targets and to provide more regular surface assemblies of antibodies.

AB - Immobilized antibodies are extensively employed for medical diagnostics such as in enzyme-linked immunosorbent assays. Despite their widespread use the ability to control the orientation on surfaces of immobilized antibodies is very limited. Herein, we report a method for covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNA origami structures. Two tris(NTA) modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysines. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) validated efficient antibody immobilization in the origami structures. The increased ability to control the orientation of antibodies in nanostructures and at surfaces has potential for directing the interactions of antibodies with targets and to provide more regular surface assemblies of antibodies.

U2 - 10.1002/anie.201706765

DO - 10.1002/anie.201706765

M3 - Journal article

VL - 56

SP - 14423

EP - 14427

JO - Angewandte Chemie (International Edition)

T2 - Angewandte Chemie (International Edition)

JF - Angewandte Chemie (International Edition)

SN - 1433-7851

IS - 46

ER -