Barcoding of soil invertebrates


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Since it was proposed to identify species by small sequences of DNA with e.g. less than 1000 bp (base pairs) popularized by the term barcode, future monitoring of biodiversity was envisaged to include barcoding (Hebert et al., 2003; Hogg and Hebert, 2004; Rougerie et al., 2009). It is now a rapidly increasing collectively endeavour supported by the infrastructure of the iBOL project (the International Barcode of Life project). By supporting the iBOL project we contribute to the study of the population genetic level of biodiversity. The barcoding is performed in collaboration with the Spatial Foodweb Ecology Group, University of Helsinki within the programme “DNA Barcode Library for (Northeast) Greenland”. NGS (Next Generation Sequencing) is under development to be employed for metagenomics and metabarcoding but in particular to be use with eDNA for determination of soil invertebrate biodiversity.

Hebert, P.D.N., Cywinska, A., Ball, S.L., deWaard, J.R., 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences 270, 313-321.

Hogg, I.D., Hebert, P.D.N., 2004. Biological identification of springtails (Hexapoda : Collembola) from the Canadian Arctic, using mitochondrial DNA barcodes. Canadian Journal of Zoology-Revue Canadienne De Zoologie 82, 749-754.

Rougerie, R., Decaëns, T., Deharveng, L., Porco, D., James, S.W., Chang, C.-H., Richard, B., Potapov, M., Suhardjono, Y., Hebert, P.D.N., 2009. DNA barcodes for soil animal taxonomy. Pesquisa Agropecuária Brasileira 44, 789-802.




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