Leif Østergaard

Noninvasive assessment of isocitrate dehydrogenase mutation status in cerebral gliomas by magnetic resonance spectroscopy in a clinical setting

Research output: Research - peer-reviewJournal article

DOI

  • Anna Tietze
  • Changho Choi
    Changho ChoiAdvanced Imaging Research Center – Radiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
  • Bruce Mickey
    Bruce MickeyNeurological Surgery Clinic, and.
  • Elizabeth A Maher
    Elizabeth A MaherDepartments of Internal Medicine and Neurology & Neurotherapeutics, University of Texas Southwestern Medical Center, Dallas, Texas.
  • Benedicte Parm Ulhøi
    Benedicte Parm Ulhøi
  • Ryan Sangill
  • Yasmin Lassen-Ramshad
    Yasmin Lassen-RamshadDepartment of Oncology and Radiotherapy
  • Slavka Lukacova
    Slavka LukacovaDepartment of Oncology and Radiotherapy
  • Leif Østergaard
  • Gorm von Oettingen
  • Plant Pathology and Entomology

OBJECTIVE Mutations in the isocitrate dehydrogenase (IDH) genes are of proven diagnostic and prognostic significance for cerebral gliomas. The objective of this study was to evaluate the clinical feasibility of using a recently described method for determining IDH mutation status by using magnetic resonance spectroscopy (MRS) to detect the presence of 2-hydroxyglutarate (2HG), the metabolic product of the mutant IDH enzyme. METHODS By extending imaging time by 6 minutes, the authors were able to include a point-resolved spectroscopy (PRESS) MRS sequence in their routine glioma imaging protocol. In 30 of 35 patients for whom this revised protocol was used the lesions were subsequently diagnosed histologically as gliomas. Of the remaining 5 patients, 1 had a gangliocytoma, 1 had a primary CNS lymphoma, and 3 had nonneoplastic lesions. Immunohistochemistry and/or polymerase chain reaction were used to detect the presence of IDH mutations in the glioma tissue resected. RESULTS In vivo MRS for 2HG correctly identified the IDH mutational status in 88.6% of patients. The sensitivity and specificity was 89.5% and 81.3%, respectively, when using 2 mM 2HG as threshold to discriminate IDH-mutated from wildtype tumors. Two glioblastomas that had elevated 2HG levels did not have detectable IDH mutations, and in 2 IDH-mutated gliomas 2HG was not reliably detectable. CONCLUSIONS The noninvasive determination of the IDH mutation status of a presumed glioma by means of MRS may be incorporated into a routine diagnostic imaging protocol and can be used to obtain additional information for patient care.

Original languageEnglish
JournalJournal of Neurosurgery: Spine
Pages (from-to)1-8
Number of pages8
ISSN1547-5654
DOIs
StateE-pub ahead of print - 3 Mar 2017

    Research areas

  • Journal Article

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