Inger Holme

Senior Researcher

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Inger Holme

Senior Researcher

  • Department of Molecular Biology and Genetics - Crop Genetics and Biotechnology
Postal address:
Forsøgsvej 1
4200
Slagelse
Denmark

Email: inger.holme@mbg.au.dk

Phone: +4587158238

Positions (after obtaining PhD)

2015-Permanent position as Academic Employee at Section for Crop Genetics and Biotechnology, Research Centre Flakkebjerg, MBG, AU
2004-2014Project Senior Scientist at Section for Crop Genetics and Biotechnology, Research Centre Flakkebjerg, MBG, AU
2001-2003Research Associate Professor at the Plant Breeding Section, KVL (presently KU)
2000Temporary employment as leader of the laboratory at the Plant Breeding Company, Abed (presently Nordic Seed)
1996-1999Research Assistant Professor at the Plant Breeding Section, KVL (presently KU)

Education and training

1996PhD from KU
1990Master of Science from University of Idaho, USA
1987Cand. agro. from KVL (presently KU)

Research stays abroad

1992/1993Nine months stay during my PhD-study at Laboratory of Plant Cell and Molecular Biology, University of Florida.
1990Six months employment at Washington State University
1988-1990Master student at University of Idaho, USA financed by a US assistantship.
1986/1987One year study at University of Idaho, USA, financed by Ella Olesen's Scholarship
1985Four months stay at Centre National de Recherche Scientifique, Gif sur Yvette, France. The stay was financed by a scholarship from the French Government.

Area of research


My research is concentrated on the development and implementation of new transformation technologies in barley and recently also in carrot. Currently, a major issue of my research involves the implementation of the two sequence specific nucleases TALENs and CRISPR/Cas9 for targeted mutagenesis, gene editing and site-specific insertions of transgenes. This is done in collaboration with an expect group in the US. At present, TALENs and CRISPR/Cas9 have been successfully implemented for targeted mutagenesis in barley and is now used in several barley projects at AU-Flakkebjerg.
My research also involves the development of plants according to the cisgenesis concept. Cisgenesis is a new transformation concept, which was developed with the aim of meeting the scepticism among the general public toward genetic modification. Cisgenesis is based on genetic modification using only the plants own genetic material or material from closely related species that can be intercrossed. Additionally, foreign sequences such as selection marker genes and vector-backbone sequences should be absent in the final cisgenic plant. Presently, the cisgenesis concept has successfully been implemented in barley using a barley phytase gene as candidate gene.
Existing transformation systems for barley can be used in only very few genotypes. To pursue this, my research has also involved the development of a transformation technique for the zygote in in vitro cultured barley ovules which is largely independent of genotype and also allows for transformation without selection.


Bibliometric summary

Wendt, T., Holme, I., Dockter, C., Preu, A., Thomas, W., Druka, A., ... Braumann, I. (2016). HvDep1 Is a Positive regulator of culm elongation and grain size in barley and impacts yield in an environment-dependent manner. P L o S One, 11(12), [e0168924]. DOI: 10.1371/journal.pone.0168924
Holme, I., Dionisio, G., Madsen, C. K., & Brinch-Pedersen, H. (2017). Barley HvPAPhy_a as transgene provides high and stable phytase activities in mature barley straw and in grains. Plant Biotechnology Journal, 15(4), 415-422. DOI: 10.1111/pbi.12636
Christiansen, M. W., Matthewman, C., Podzimska-Sroka, D., O'Shea, C., Lindemose, S., Møllegaard, N. E., ... Gregersen, P. L. (2016). Barley plants over-expressing the NAC transcription factor gene HvNAC005 show stunting and delay in development combined with early senescence. Journal of Experimental Botany, 67(17), 5259-5273. DOI: 10.1093/jxb/erw286
Gallage, N. J., Hansen, E. H., Kannangara, R., Olsen, C. E., Motawia, M. S., Jørgensen, K., ... Møller, B. L. (2014). Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme. Nature Communications, 5, 1-14. [4037]. DOI: 10.1038/ncomms5037
Brinch-Pedersen, H., Madsen, C. K., Holme, I. B., & Dionisio, G. (2014). Increased understanding of the cereal phytase complement for better mineral bio-availability and resource management. Journal of Cereal Science, 59(3), 373-381. DOI: 10.1016/j.jcs.2013.10.003
Chen, Y-J., Perera, V., Wagner, M., Holme, I., Gregersen, P. L., Grant, M. R., ... Lyngkjær, M. F. (2013). The barley HvNAC6 transcription factor affects ABA accumulation and promotes basal resistance against powdery mildew. Plant Molecular Biology, 83(6), 577-590. DOI: 10.1007/s11103-013-0109-1
Wendt, T., Holm, P. B., Starker, C. G., Christian, M., Voytas, D. F., Brinch-Pedersen, H., & Holme, I. (2013). TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants. Plant Molecular Biology, 83(3), 279-285. DOI: 10.1007/s11103-013-0078-4
Holme, I., Wendt, T., & Holm, P. B. (2013). Intragenesis and cisgenesis as alternatives to transgenic crop development. Plant Biotechnology Journal, 11(4), 395-407. DOI: 10.1111/pbi.12055
Madsen, C. K., Dionisio, G., Holme, I., Holm, P. B., & Brinch-Pedersen, H. (2013). High mature grain phytase activity in the Triticeae has evolved by duplication followed by neofunctionalization of the purple acid phosphatase phytase (PAPhy) gene. Journal of Experimental Botany, 64(11), 3111-3123. DOI: 10.1093/jxb/ert116
Holme, I., Dionisio, G., Brinch-Pedersen, H., Wendt, T., Madsen, C. K., Vincze, É., & Holm, P. B. (2012). Cisgenic barley with improved phytase activity. Plant Biotechnology Journal, 10(2), 237-247. DOI: 10.1111/j.1467-7652.2011.00660.x
Holme, I., Brinch-Pedersen, H., Lange, M., & Holm, P. B. (2012). Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules. Methods in Molecular Biology, 847(4), 151-161. DOI: 10.1007/978-1-61779-558-9_13
Kichey, T., Holme, I., Møller, I. S., Jahn, T. P., Holm, P. B., & Schjoerring, J. K. (2009). Improving nitrogen use efficiency in barley (Hordeum vulgare L.) through the cisgenic approach. In The Proceedings of the International Plant Nutrition Colloquium XVI Department of Plant Sciences, University of California, Davis.
Holme, I. B., Brinch-Pedersen, H., Lange, M., & Holm, P. B. (2008). Transformation of different barley (Hordeum vulgare L.) cultivars by Agrobacterium tumefaciens infection of in vitro cultured ovules. Plant Cell Reports, 27(12), 1833-1840. DOI: 10.1007/s00299-008-0605-y
Louarn, S., Torp, A. M., Holme, I., Andersen, S. B., & Jensen, B. D. (2007). Database derived microsatellite markers (SSRs) for cultivar differentiation in Brassica oleracea. Genetic Resources and Crop Evolution, 54(8), 1717-1725. DOI: 10.1007/s10722-006-9181-6
Holme, I. B., Brinch-Pedersen, H., Lange, M., & Holm, P. B. (2006). Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules. Plant Cell Reports, 25(12), 1325-1335. DOI: 10.1007/s00299-006-0188-4
Holme, I. B., Torp, A. M., Hansen, L. N., & Andersen, S. B. (2004). Quantitative trait loci affecting protoplast regeneration in Brassica oleracea. Theoretical and Applied Genetics, 108(8), 1513-1520. DOI: 10.1007/s00122-003-1570-z
Holme, I. B., Olesen, A., Hansen, N. J. P., & Andersen, S. B. (1999). Anther and isolated microspore culture of wheat lines from northwestern and eastern Europe. Plant Breeding, 118, 111-117. DOI: 10.1111/j.1439-0523.1999.tb01499.x
Holme, I. B. (1998). Growth characteristics and nutrient depletion of Miscanthus x ogiformis Honda 'Giganteus' suspension cultures. Plant Cell, Tissue and Organ Culture, 53, 143-151. DOI: 10.1023/A:1006001419989
Holme, I. B., Krogstrup, P., & Hansen, J. (1997). Embryogenic callus formation, growth and regeneration in callus and suspension cultures of Miscanthus x ogiformis Honda 'Giganteus' as affected by proline. Plant Cell, Tissue and Organ Culture, 50, 203-210. DOI: 10.1023/A:1005981300847
Holme, I. B., & Petersen, K. K. (1996). Callus induction and plant regeneration from different explant types of Miscanthus x ogiformis Honda 'Giganteus'. Plant Cell, Tissue and Organ Culture, 45, 43-52. DOI: 10.1007/BF00043427

Invited speaker at international scientific congresses

I.B. Holme (2014). Targeted mutagenesis using TALENs as means to improve cereal grain quality. AACC International Annual Meeting, Providence, Rhode Island, USA.
I.B. Holme (2014). Development of cisgenic barley. PlantTransformationTechnologies III, Vienna, Austria
I.B. Holme (2012). Cisgenic barley. Minisymposium on: Cisgenesis, next step in classical plant breeding. Wageningen University, The Netherlands.
I.B. Holme (2008). Cisgenic barley for animal feed. Second Workshop on TritiGen COST Action FA0604. Bulgaria.
I.B. Holme (2008). Cisgenic barley for animal feed. Plant Genomics European Meeting 2008. Bulgaria.
I.B. Holme (2007). Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules. Meeting at Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

International collaborations

Centre for Genome Engineering at University of Minnesota, USA.

Research funding

Research project: (Grant provider: Fødevareforskningsprogrammet 2009 / Principal applicant: Inger Bæksted Holme / Title: New techniques for genetic modification of barley and wheat / Amount: 7.8 million Dkr / My share: 7.8 million Dkr / Period: 2010-2013).
Research project: (Grant provider: Brd. Hartmann fond / Principal applicant:
Inger Bæksted Holme / Title: Målrettet mutagenese i byg / Amount: 50.000 Dkr / My share: 50.000 Dkr / Period: 2015-2016)

Teaching and supervision

Pedagogical training 1998/1999:

Completed the one-year study programme in educational studies for assistant professors at KVL (presently KU).

Teaching experience:

Lectures and supervision of different undergraduate and graduate exercise courses related to plant breeding and plant genetic transformation at KU and AU, respectively.

Supervision of students:

Former co-supervisor at AU for the PhD students: Michael Wagner, 2008-2011 and Dagmara Podzimska, 2011-2015. Presently co-supervisor at AU for the PhD students: Shahin Noeparvar, Shrikant Sharma and Michael Panting.

Post doctoral training:

I am currently supervising postdoc Dagmara Podzimska.


Evaluation of others work

I am frequently a reviewer on scientific journals and on international grants. Occasionally, I am also external examiner on PhD defences abroad.