Rasmus O. Bak

Associate professor, AIAS-COFUND Fellow

Rasmus Bak
See relations at Aarhus University

Profile

I am a research scientist with a strong background in developing strategies and applying novel techniques for genome editing in human cells. I have 10 years of research experience during which I have worked with a wide panel of gene expression vectors, such as plasmids, transposons, lentiviral vectors, and adeno-associated viral vectors. In addition, I have worked with several genome editing platforms including zing finger nucleases (ZFNs), TALE nucleases (TALENs), and CRISPR/Cas9. I have developed chemically modified guide RNAs for use in the CRISPR/Cas9 system and shown that they can facilitate very high levels of genome editing in primary human hematopoietic stem and progenitor cells (Nature Biotechnology, Sept. 2015). I used this platform to develop a potent CRISPR/Cas9-based genome editing strategy for Sickle Cell Disease (Nature, Nov. 2016; Nature Protocols, Jan. 2018). I have also demonstrated that the system supports integration of large transgenes by sequential homologous recombination (Cell Reports, July 2017) and multiplexed genome editing (eLife, Sept. 2017).

  • Published

    A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells

    Vakulskas, C. A., Dever, D. P., Rettig, G. R., Turk, R., Jacobi, A. M., Collingwood, M. A., Bode, N. M., McNeill, M. S., Yan, S., Camarena, J., Lee, C. M., Park, S. H., Wiebking, V., Bak, R. O., Gomez-Ospina, N., Pavel-Dinu, M., Sun, W., Bao, G., Porteus, M. H. & Behlke, M. A., 2018, In : Nature Medicine. 24, 8, p. 1216-1224 9 p.

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Published

    CRISPR/Cas9 genome editing in human hematopoietic stem cells

    Bak, R., Dever, D. P. & Porteus, M. H., 2018, In : Nature Protocols (Print). 13, 2, p. 358-376 19 p.

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Published

    CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells

    Dever, D. P., Bak, R. O., Reinisch, A., Camarena, J., Washington, G., Nicolas, C. E., Pavel-Dinu, M., Saxena, N., Wilkens, A. B., Mantri, S., Uchida, N., Hendel, A., Narla, A., Majeti, R., Weinberg, K. I. & Porteus, M. H., 17 Nov 2016, In : Nature. 539, 7629, p. 384-389 6 p.

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Published

    Activation of proto-oncogenes by disruption of chromosome neighborhoods

    Hnisz, D., Weintraub, A. S., Day, D. S., Valton, A-L., Bak, R. O., Li, C. H., Goldmann, J., Lajoie, B. R., Fan, Z. P., Sigova, A. A., Reddy, J., Borges-Rivera, D., Lee, T. I., Jaenisch, R., Porteus, M. H., Dekker, J. & Young, R. A., 25 Mar 2016, In : Science. 351, 6280, p. 1454-1458 5 p.

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Published

    Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

    Hendel, A., Bak, R. O., Clark, J. T., Kennedy, A. B., Ryan, D. E., Roy, S., Steinfeld, I., Lunstad, B. D., Kaiser, R. J., Wilkens, A. B., Bacchetta, R., Tsalenko, A., Dellinger, D., Bruhn, L. & Porteus, M. H., 29 Jun 2015, In : Nature Biotechnology.

    Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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