Increase in circulating holotranscobalamin after oral administration of cyanocobalamin or hydroxocobalamin in healthy adults with low and normal cobalamin status

Publikation: Forskning - peer reviewTidsskriftartikel

DOI

  • Eva Greibe
  • Namita Mahalle
    Namita MahalleDepartment of Pathology, Deenanath Mangeshkar Hospital and Research Center, PuneIndien
  • Vijayshri Bhide
    Vijayshri BhideDepartment of Pathology, Deenanath Mangeshkar Hospital and Research Center, PuneIndien
  • Christian Würtz Heegaard
  • Sadanand Naik
    Sadanand NaikDepartment of Pathology, Deenanath Mangeshkar Hospital and Research Center, PuneIndien
  • Ebba Nexø
PURPOSE:
To investigate the absorption of synthetic cyanocobalamin and natural occurring hydroxocobalamin in populations with low and normal cobalamin (vitamin B12) status.

METHODS:
We included adults with low (n = 59) and normal (n = 42) cobalamin status and measured the change in serum holotranscobalamin (ΔholoTC) before and after 2 day administration of different doses of cyanocobalamin and hydroxocobalamin (CobaSorb test). In the low status group, the test was performed using a cross-over design with identical doses of both cobalamin forms (1.5, 3, and 6 µg, respectively). In the normal status group, the test was performed with either 3, 6, and 9 µg cyanocobalamin (n = 28), or with 9 µg cyanocobalamin and 9 µg hydroxocobalamin (n = 14).

RESULTS:
In both groups, median ΔholoTC (pmol/L) was higher after intake of cyanocobalamin compared to (hydroxocobalamin) [low status: 1.5 µg: 19 (6); 3 µg: 23 (7); 6 µg: 30 (14); normal status: 9 µg: 30 (13) pmol/L]. Independent of B12 form, no difference was observed in ΔholoTC between those receiving 1.5 and 3 µg in the low status group or 6 and 9 µg cyanocobalamin in the normal status group. However, in both groups, administration of 6 µg cobalamin resulted in a significant higher ΔholoTC than did 3 µg [low status: p = 0.02 (0.009) for cyanocobalamin (hydroxocobalamin); normal status: p = 0.03 for cyanocobalamin].

CONCLUSIONS:
Administration of cyanocobalamin resulted in a more than twofold increase in holoTC in comparison with hydroxocobalamin. The absorptive capacity was reached only by doses above 3 µg cobalamin. Our results underscore the importance of using the same form of cobalamin when comparing uptake under different conditions.
OriginalsprogEngelsk
TidsskriftEuropean Journal of Nutrition
Vol/bind[Epud ahead of print]
Antal sider9
ISSN1436-6207
DOI
StatusUdgivet - 16 okt. 2017

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