Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

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Dokumenter

DOI

  • Ilenia Manuguerra
  • Stefano Croce, Univeristy of Rome, Tor Vergata, Departmen of chemistry, Ludwig-Maximilians-Universität, Munich, ItalienAfaf H. El-Sagheer, Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Abhichart Krissanaprasit, Tom Brown, Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Storbritannien
  • Kurt Vesterager Gothelf
  • Antonio Manetto, Metabion GmbH, Tyskland
Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.
OriginalsprogEngelsk
TidsskriftChemical Communications
Vol/bind54
Antal sider4
ISSN1359-7345
DOI
StatusE-pub ahead of print - 20 mar. 2018

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