Lotus japonicus NOOT-BOP-COCH-LIKE1 is essential for nodule, nectary, leaf and flower development

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DOI

    Kévin Magne, Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France., Jeoffrey George, Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France., Ana Berbel Tornero, Instituto de Biología Molecular y Celular de Plantas, CSIC-UPV, Universidad Politécnica de Valencia, CPI Edificio 8E, Avenida de los Naranjos s/n, Valencia, 46022, Spain., Blandine Broquet, Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France., Francisco Madueño, Instituto de Biología Molecular y Celular de Plantas, CSIC-UPV, Universidad Politécnica de Valencia, CPI Edificio 8E, Avenida de los Naranjos s/n, Valencia, 46022, Spain.,
  • Stig Uggerhøj Andersen
  • Pascal Ratet, Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France.

The NOOT-BOP-COCH-LIKE (NBCL) genes are orthologs of Arabidopsis thaliana BLADE-ON-PETIOLE1/2. NBCLs are developmental regulators essential for plant shaping mainly through the regulation of organ boundaries, the promotion of lateral organ differentiation and the acquisition of organ identity. In addition to their roles in leaf, stipule and flower development, NBCLs are required for maintaining the identity of indeterminate nitrogen fixing nodules with persistent meristems in legumes. In legume forming determinate nodules without persistent meristem, the roles of the NBCL genes are not known. We thus investigated the role of the Lotus japonicus LjNOOT-BOP-COCH-LIKE1 (LjNBCL1) in determinate nodule identity and studied its functions in aerial organ development using LORE1 insertional mutants and RNAi-mediated silencing approaches. In Lotus, LjNBCL1 is involved in leaf patterning and participates in the regulation of axillary outgrowth. Wild-type Lotus leaves are composed of five leaflets and possess a pair of nectaries at the leaf axil. Legumes such as pea and Medicago have a pair of stipules at the base of their leaves, rather than nectaries. In Ljnbcl1, nectaries development is abolished, demonstrating that nectaries and stipules share a common evolutionary origin. In addition, ectopic roots arising from nodule vascular meristems and re-organization of the nodule vascular bundle vessels were observed on Ljnbcl1 nodules. This demonstrates that NBCL functions are conserved in both indeterminate and determinate nodules through the maintenance of nodule vascular bundle identity. In contrast to their roles in floral patterning described in others plants, LjNBCL1 appears essential for both secondary inflorescence meristem and floral meristem development. This article is protected by copyright. All rights reserved.

OriginalsprogEngelsk
TidsskriftPlant Journal
Vol/bind94
Sider (fra-til)880-894
Antal sider15
ISSN0960-7412
DOI
StatusUdgivet - jun. 2018

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