Silver (nano)materials cause genotoxicity in Enchytraeus crypticus, as determined by the comet assay

Publikation: Forskning - peer reviewTidsskriftartikel

DOI

  • Vera L Maria
    Vera L MariaDepartment of Biology & CESAM, University of Aveiro, Aveiro, Portugal
  • Maria João Ribeiro
  • Sofia Guilherme
    Sofia GuilhermeDepartment of Biology & CESAM, University of Aveiro, Aveiro, Portugal
  • Amadeu M V M Soares
    Amadeu M V M SoaresDepartment of Biology & CESAM, University of Aveiro, Aveiro, Portugal
  • Janeck J Scott-Fordsmand
  • Mónica J B Amorim
    Mónica J B AmorimDepartment of Biology & CESAM, University of Aveiro, Aveiro, Portugal

Enchytraeids have been used in standard ecotoxicity testing for about 20 years now. Since adopting the standard test for survival and reproduction, a number of additional tools have been developed, including transcriptomics and enzymatic biomarkers. So far, a genotoxicity tool and endpoint have not been used; hence, the goals of the current study included the optimization of the in vivo alkaline comet assay in Enchytraeus crypticus. Further, the effect of silver nanomaterial (Ag NM300K, dispersed, 15 nm) was tested and compared with AgNO3 . Hydrogen peroxide (H2 O2 ) was used as a positive control. The various steps were optimized. The fully detailed standard operating procedure (SOP) is presented here. Ag materials caused genotoxicity, this being differentiated for the nano and non-nano forms. AgNO3 caused genotoxicity after 3 days (d) of exposure in a dose related manner, although after 7d the effects were either reduced or repaired, whereas Ag NM300K caused higher genotoxicity after 7d for the lowest concentration (EC20), highlighting a potential non-monotone dose-response effect. Overall, the comet assay showed the power to discriminate effects between materials and also toxicity at low relevant doses. This article is protected by copyright. All rights reserved.

OriginalsprogEngelsk
TidsskriftEnvironmental Toxicology and Chemistry
Vol/bind37
Tidsskriftsnummer1
Sider (fra-til)184-191
ISSN0730-7268
DOI
StatusUdgivet - jan. 2018

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