The polymorphic insertion of the luteinizing hormone receptor "insLQ" show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles

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DOI

  • T Borgbo
  • J Chrudimska, Department of Biology and Medical Genetics, 2nd Faculty of Medicine Charles University, University Hospital Motol, Prague, Czech Republic., M Macek, Department of Biology and Medical Genetics, 2nd Faculty of Medicine Charles University, University Hospital Motol, Prague, Czech Republic., J V Jeppesen, Laboratory of Reproductive Biology, University Hospital of Copenhagen (Rigshospitalet), Copenhagen Ø, Denmark., Jane Alrø Bøtkjær, Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark. yding@rh.dk, S G Kristensen, Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark. yding@rh.dk, K T Macklon, The Fertility Clinic, The JulianeMarie Centre, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark.,
  • E Ernst
  • L L Hansen
  • C. Yding Andersen, Laboratory of Reproductive Biology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark. Electronic address: claus.yding.andersen@regionh.dk.
The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Müllerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to -/insLQ and the -/- genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in -/insLQ compared to the -/- genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.
OriginalsprogEngelsk
TidsskriftMolecular and Cellular Endocrinology
Vol/bind460
Sider (fra-til)57-62
Antal sider6
ISSN0303-7207
DOI
StatusUdgivet - 15 jan. 2018

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