Developmental competence and epigenetic profile of porcine embryos produced by two different cloning methods

Publikation: Forskning - peer reviewTidsskriftartikel

DOI

  • Ying Liu
    Ying Liu
  • Andrea Lucas-Hahn
    Andrea Lucas-HahnInstitute of Farm Animal Genetics (FLI)Tyskland
  • Bjoern Petersen
    Bjoern PetersenInstitute of Farm Animal Genetics, FLITyskland
  • Rong Li
    Rong Li
  • Doris Hermann
    Doris HermannInstitute for Farm Animal GeneticsTyskland
  • Petra Hassel
    Petra HasselInstitute for Farm Animal GeneticsTyskland
  • Maren Ziegler
    Maren ZieglerInstitute for Farm Animal GeneticsTyskland
  • Knud Erik Larsen
  • Heiner Niemann
    Heiner NiemannInstitute of Farm Animal Genetics, FLITyskland
  • Henrik Callesen
The “Dolly” based cloning (classical nuclear transfer, [CNT]) and the handmade cloning (HMC) are methods that are nowadays routinely used for somatic cloning of large domestic species. Both cloning protocols share several similarities, but differ with regard to the required in vitro culture, which in turn results in different time intervals until embryo transfer. It is not yet known whether the differences between cloned embryos from the two protocols are due to the cloning methods themselves or the in vitro culture, as some studies have shown detrimental effects of in vitro culture on conventionally produced embryos. The goal of this study was to unravel putative differences between two cloning methods, with regard to developmental competence, expression profile of a panel of developmentally important genes and epigenetic profile of porcine cloned embryos produced by either CNT or HMC, either with (D5 or D6) or without (D0) in vitro culture. Embryos cloned by these two methods had a similar morphological appearance on D0, but displayed different cleavage rates and different quality of blastocysts, with HMC embryos showing higher blastocyst rates (HMC vs. CNT: 35% vs. 10%, p < 0.05) and cell numbers per blastocyst (HMC vs. CNT: 31 vs. 23 on D5 and 42 vs. 18 on D6, p < 0.05) compared to CNT embryos. With regard to histone acetylation and gene expression, CNT and HMC derived cloned embryos were similar on D0, but differed on D6. In conclusion, both cloning methods and the in vitro culture may affect porcine embryo development and epigenetic profile. The two cloning methods essentially produce embryos of similar quality on D0 and after 5 days in vitro culture, but thereafter both histone acetylation and gene expression differ between the two types of cloned embryos.
OriginalsprogEngelsk
TidsskriftCellular Reprogramming
Vol/bind19
Tidsskriftsnummer3
Sider (fra-til)171-179
Antal sider9
ISSN2152-4971
DOI
StatusUdgivet - jun. 2017

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