Transport of valine across the small intestinal epithelium in pigs fed different valine levels and Bacillus subtilis

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DOI

    K Blaabjerg,
  • J V Nørgaard
  • B Nielsen, Bacterial Physiology and Improvement, R&D Microbial Platform, Chr. Hansen A/S, Hørsholm, Denmark., M D Cantor, Bacterial Physiology and Improvement, R&D Microbial Platform, Chr. Hansen A/S, Hørsholm, Denmark., P Derkx, Bacterial Physiology and Improvement, R&D Microbial Platform, Chr. Hansen A/S, Hørsholm, Denmark.,
  • J Sehested
  • H D Poulsen
Mutants of Bacillus subtilis overproducing valine (B. subtilis VAL) could be an approach to supply pigs dietary valine (Val). In the study, 18 gilts were fed: (i) negative diet with a standardized ileal digestible (SID) Val:Lys of 0.63:1 (Neg); (ii) Neg added B. subtilis VAL (1.28 × 1011 cfu/kg as‐fed) or; (iii) Neg added L‐Val to a Val:Lys of 0.69:1. Using the Ussing chamber method, the study aimed to investigate whether (i) the diets affect intestinal transport of additions of 0, 5, 10 or 20 mmol Val/L from the mucosal to the serosal side and (ii) the B. subtilis VAL contributes to a net transport of Val produced in situ. The results showed that the Isc (ΔIscVal) and release of Val to the serosal side solution (Srel; μmol cm−2 min−1) increased with Val addition (linear and quadratic, p < .0001) but was similar for 5, 10 or 20 mmol Val/L and not affected by diet. No net transport of in situ produced Val by B. subtilis VAL was detected. In conclusion, feeding a Val‐deficient diet with or without B. subtilis VAL or a Val sufficient diet did not affect the Val transport across intestinal epithelia. No in situ Val production by B. subtilis VAL was observed in the Ussing chambers.
OriginalsprogEngelsk
TidsskriftJournal of Animal Physiology and Animal Nutrition
Vol/bind102
Tidsskriftsnummer2
Sider (fra-til)e856-e863
ISSN0931-2439
DOI
StatusUdgivet - apr. 2018

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