Effects of post-mortem storage conditions of bovine epididymides on sperm characteristics: investigating a tool for preservation of sperm from endangered species.

Publikation: Forskning - peer reviewTidsskriftartikel

DOI

  • Julie Strand
    Julie StrandDepartment of chemistry and bioscience, Section of Biology and Environmental Science, Aalborg UniversityDanmark
  • Mette M Ragborg
    Mette M RagborgDepartment of chemistry and bioscience, Section of Biology and Environmental Science, Aalborg UniversityDanmark
  • Hanne Skovsgaard Pedersen
  • Torsten Nygård Kristensen
  • Cino Pertoldi
    Cino PertoldiDept of Biotechnology, Chemistry and Environmental Engineering, Aalborg UniversityDanmark
  • Henrik Callesen
The aim of this study was to establish and validate a reliable and efficient protocol for the recovery and cryopreservation of epididymal spermatozoa used for in vitro fertilization, using bulls of two different age classes. Testicles from 26 (37–51 weeks old, group 1) and 19 (52–115 weeks old, group 2) Danish Holstein bulls were collected after slaughter and stored at 5°C. After 0, 24 or 48 h, epididymides were isolated and spermatozoa collected. Assessments included spermatozoal motility, viability and morphology before and after cryopreservation and in vitro embryo production. Results showed that live spermatozoa can be collected from epididymides of bulls after their death. Storage of the testicles at 5°C for 24 h followed by cryopreservation of recovered epididymal spermatozoa resulted in 21% (group 1) and 31% (group 2) blastocysts produced in vitro. These results illustrate that epididymal spermatozoa recovered from testicles kept in specific conditions can be used to preserve genetic material from endangered and threatened species or populations in nature as well as in domestic and zoo animals.
OriginalsprogEngelsk
Artikelnummercow069
TidsskriftConservation Physiology
Vol/bind4
Tidsskriftsnummer1
Antal sider8
ISSN2051-1434
DOI
StatusUdgivet - 29 dec. 2016

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