Evaluation of a gas in vitro system for predicting methane production in vivo

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikel

Dokumenter

DOI

    Rebecca Danielsson, Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, SE-75323, Uppsala, Sweden, SverigeMohammad Ramin, Department of Agricultural Research for Northern Sweden, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden, SverigeJan Bertilsson, Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, SE-75323, Uppsala, Sweden, Sverige
  • Peter Lund
  • Pekka Huhtanen, Department of Agricultural Research for Northern Sweden, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden, Sverige
Methane production from ruminant livestock varies with the diet as a result of factors such as dry matter intake, diet composition, and digestibility. To estimate the effect of dietary composition and feed additives, CH4 production can be measured in vitro as a first step because large numbers of samples can be incubated and analyzed at the same time. This study evaluated a recently developed in vitro method for prediction of in vivo CH4 production by examining the relationship between predicted and observed CH4 production values. A total of 49 different diets (observations), used in previous 13 in vivo studies, were selected to include diets varying in nutrient composition. Methane production was measured in all in vivo studies by respiration chambers or the GreenFeed system (C-Lock Inc., Rapid City, SD). Overall, the in vitro system predicted CH4 production well (R2 = 0.96), but the values obtained were slightly underestimated compared with observed in vivo values (mean 399 L/d compared with 418 L/d: root mean square prediction error = 51.6 L/d or 12.3% of observed mean). Further analysis of the effect on residuals showed no significant relationship between CH4 production and most factors known to affect CH4 production such as dry matter intake, digestibility, and dietary concentrations of fat and starch. However, some factors included in the model were not well predicted by the system, with residuals negatively related to neutral detergent fiber concentration and positively related to concentrate proportion. The in vitro system can thus be useful for screening diets and evaluation of feed additives as a first step that can be best interpreted when feeding cows at maintenance level.
OriginalsprogEngelsk
TidsskriftJournal of Dairy Science
Vol/bind100
Tidsskriftsnummer11
Sider (fra-til)8881-8894
ISSN0022-0302
DOI
StatusUdgivet - 6 sep. 2017

Se relationer på Aarhus Universitet Citationsformater

ID: 120114485