Fast Screening of Antibacterial Compounds from Fusaria

Publikation: Forskning - peer reviewTidsskriftartikel

Dokumenter

DOI

  • Teis Esben Sondergaard
    Teis Esben SondergaardAalborg Univ, Aalborg University, Dept Chem & Biosci
  • Marlene Fredborg
  • Ann-Maria Oppenhagen Christensen
    Ann-Maria Oppenhagen ChristensenAalborg Univ, Aalborg University, Dept Chem & Biosci
  • Sofie K. Damsgaard
    Sofie K. DamsgaardAalborg Univ, Aalborg University, Dept Chem & Biosci
  • Nikoline F. Kramer
    Nikoline F. KramerAalborg Univ, Aalborg University, Dept Chem & Biosci
  • Henriette Giese
    Henriette GieseAalborg Univ, Aalborg University, Dept Chem & Biosci
  • Jens Laurids Sorensen
    Jens Laurids SorensenAalborg Univ, Aalborg University, Dept Chem & Biosci
Bio-guided screening is an important method to identify bioactive compounds from fungi. In this study we applied a fast digital time-lapse microscopic method for assessment of the antibacterial properties of secondary metabolites from the fungal genus Fusarium. Here antibacterial effects could be detected for antibiotic Y, aurofusarin, beauvericin, enniatins and fusaric acid after six hours of cultivation. The system was then used in a bio-guided screen of extracts from 14 different Fusarium species, which had been fractionated by HPLC. In this screen, fractions containing the red pigments aurofusarin and bikaverin showed effects against strains of Lactobacillus and Bifidobacterium. The IC50 for aurofusarin against Lactobacillus acidophilus was 8 mu M, and against Bifidobacterium breve it was 64 mu M. Aurofusarin only showed an effect on probiotic bacteria, leading to the speculation that only health-promoting bacteria with a positive effect in the gut system are affected.
OriginalsprogEngelsk
Artikelnummer355
TidsskriftToxins
Vol/bind8
Tidsskriftsnummer12
Antal sider9
ISSN2072-6651
DOI
StatusUdgivet - 29 nov. 2016

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