Comparing the desorption and biodegradation of low concentrations of phenanthrene sorbed to activated carbon, biochar and compost

Publikation: Forskning - peer reviewTidsskriftartikel

  • Geoffrey Marchal
    Geoffrey MarchalDanmark
  • Kilian E. C. Smith
    Kilian E. C. SmithDanmark
  • Arno Rein
    Arno ReinDepartment of Environmental Engineering, Technical University of Denmark
  • Anne Winding
  • Stefan Trapp
    Stefan TrappDepartment of Environmental Engineering, Technical University of Denmark
  • Ulrich Bay Gosewinkel
Carbonaceous soil amendments are applied to contaminated soils and sediments to strongly sorb hydrophobic organic contaminants (HOCs) and reduce their freely dissolved concentrations. This limits biouptake and toxicity, but also biodegradation. To investigate whether HOCs sorbed to such amendments can
be degraded at all, the desorption and biodegradation of low concentrations of 14C-labelled phenanthrene (65 lg L1) freshly sorbed to suspensions of the pure soil amendments activated carbon (AC), biochar (charcoal) and compost were compared. Firstly, the maximum abiotic desorption of phenanthrene from
soil amendment suspensions in water, minimal salts medium (MSM) or tryptic soy broth (TSB) into a dominating silicone sink were measured. Highest fractions remained sorbed to AC (84 ± 2.3%, 87 ± 4.1%, and 53 ± 1.2% for water, MSM and TSB, respectively), followed by charcoal (35 ± 2.2%, 32 ± 1.7%, and 12 ± 0.3%, respectively) and compost (1.3 ± 0.21%, similar for all media). Secondly, the mineralization of phenanthrene sorbed to AC, charcoal and compost by Sphingomonas sp. 10-1 (DSM 12247) was determined. In contrast to the amounts desorbed, phenanthrene mineralization was similar for all
the soil amendments at about 56 ± 11% of the initially applied radioactivity. Furthermore, HPLC analyses showed only minor amounts (<5%) of residual phenanthrene remaining in the suspensions, indicating almost complete biodegradation. Fitting the data to a coupled desorption and biodegradation model revealed that desorption did not limit biodegradation for any of the amendments, and that degradation could proceed due to the high numbers of bacteria and/or the production of biosurfactants or biofilms. Therefore, reduced desorption of phenanthrene from AC or charcoal did not inhibit its iodegradation,
which implies that under the experimental conditions these amendments can reduce freely dissolved concentration without hindering biodegradation. In contrast, phenanthrene sorbed to compost was fully desorbed and biodegraded.
OriginalsprogEngelsk
TidsskriftChemosphere
Vol/bind90
Sider (fra-til)1767-1778
ISSN0045-6535
DOI
StatusUdgivet - 2013

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