Monitoring soil bacteria with community-level physiological profiles using Biolog™ ECO-plates in the Netherlands and Europe

Publikation: Forskning - peer reviewTidsskriftartikel

  • Michiel Rutgers
    Michiel RutgersNational Institute for Public Health and the Environment (RIVM), Bilthoven
  • Marja Wouterse
    Marja WouterseNational Institute for Public Health and the Environment (RIVM), BilthovenDanmark
  • Sytske M. Drost
    Sytske M. DrostNational Institute for Public Health and the Environment (RIVM), BilthovenDanmark
  • Anton M. Breure
    Anton M. BreureNational Institute for Public Health and the Environment (RIVM)Department of Environmental Science, Institute for Water and Wetland Research (IWWR), Radboud University NijmegenDanmark
  • Christian Mulder
    Christian MulderNational Institute for Public Health and the Environment (RIVM), Bilthoven
  • Dorothy Stone
    Dorothy StoneSoils and Land Use Research DepartmentLeeds UniversityStorbritannien
  • Rachel E. Creamer
    Rachel E. CreamerSoils and Land Use Research DepartmentDanmark
  • Anne Winding
  • Jaap Bloem
    Jaap BloemWageningen University and Research Centre
Soil samples were analyzed with community-level physiological profiles (CLPP) using Biolog™ ECO-plates in the Netherlands Soil Monitoring Network (NSMN; 704 samples) and in a European-wide transect (73 samples). The selection of sites was based on a representative sample of major soil texture types, land uses and climate zones. This method for determination of CLPP was specifically designed to be applied in large and long-term monitoring activities. It encompassed a solution for the inoculum-density dependence of colour formation patterns in ECO-plates, through stepwise dilution to extinction of the bacterial inoculum. The CLPP in Dutch and European soil samples appeared to be reproducible and sensitive to land use and/or soil texture. Although the method is selective, CLPP based parameters correlated well with other microbial parameters and soil characteristics. Consistent patterns in CLPP and soil habitat characteristics are emerging, as brought about by environmental disturbances, land management and soil texture. The applicability of CLPP analysis in monitoring systems is discussed.
OriginalsprogEngelsk
TidsskriftApplied Soil Ecology
Vol/bind97
Sider (fra-til)23–35
Antal sider13
ISSN0929-1393
DOI
StatusUdgivet - jan. 2016

    Forskningsområder

  • Biolog, Indicator, Method, Microbial community, MicroResp, Soil

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