Extrachromosomal deoxyribonucleic acid in R factor-harboring Enterobacteriaceae.

Publikation: Forskning - peer reviewTidsskriftartikel

  • JK Møller
    JK MøllerDanmark
  • AL Bak
    AL BakDanmark
  • C Christiansen
    C ChristiansenDanmark
  • Gunna Christiansen
  • A. Stenderup
    A. StenderupDanmark
  • Institut for Medicinsk Mikrobiologi og Immunologi

Extrachromosomal deoxyribonucleic acid (DNA) from 24 different R factor-harboring Enterobacteriaceae was isolated and characterized by analytical ultracentrifugation and electron microscopy. The R factors represented 15 different patterns of transferable drug resistance found in enterobacteria from an enclosed geographic area. All of the strains contained extrachromosomal, circular DNA molecules within the range of 0.4 to 52 mum. More than one size class of circular DNA molecules was observed in the majority of the extrachromosomal DNA preparations. The buoyant density of the extrachromosomal DNA ranged from 1.700 to 1.720 g/cm3. The majority of the bacteria contained extrachromosomal DNAs of various densities. Three-fourths of the R factors were classified as fi+. The investigation illustrates the extensive variability in the physical characteristics of plasmid DNA from R factor-harboring strains.

OriginalsprogEngelsk
TidsskriftJ Bacteriol
Vol/bind125
Sider (fra-til)398-403
StatusUdgivet - 1976

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