Differential impact of glucose administered intravenously and orally on circulating miR-375 levels in human subjects

Publikation: Forskning - peer reviewTidsskriftartikel

DOI

  • Xin Yan
    Xin YanMax-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Strasse 10, 13125, Berlin, Germany.
  • Zhen Wang
    Zhen WangMax-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Strasse 10, 13125, Berlin, Germany.
  • Sidse Westberg-Rasmussen
    Sidse Westberg-RasmussenInstitut for Klinisk Medicin - Medicinsk Endokrinologisk afdeling MEA
  • Marcel Tarbier
    Marcel TarbierScience for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 17121 Stockholm, Sweden.
  • Thomas Rathjen
    Thomas RathjenMax-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Strasse 10, 13125, Berlin, Germany.
  • Sudhir G Tattikota
    Sudhir G TattikotaMax-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Strasse 10, 13125, Berlin, Germany.
  • Bailey C E Peck
    Bailey C E PeckDepartment of Surgery, School of Medicine, University of Michigan, Ann Arbor, Michigan 48109.
  • Matt Kanke
    Matt KankeDepartment of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.
  • Claus Oxvig
  • Jan Frystyk
  • Jakob Starup-Linde
  • Praveen Sethupathy
    Praveen SethupathyDepartment of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.
  • Marc R Friedländer
    Marc R FriedländerScience for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 17121 Stockholm, Sweden.
  • Søren Gregersen
    Søren Gregersen
  • Matthew N Poy
    Matthew N PoyMax-Delbrueck-Center for Molecular Medicine, Robert-Roessle-Strasse 10, 13125, Berlin, Germany.

Background: To date, numerous nucleic acid species have been detected in the systemic circulation including microRNAs (miRNAs); however their functional role in this compartment remains unclear.

Objective: The aim of this study was to determine whether systemic levels of miRNAs abundant in blood, including the neuroendocrine tissue-enriched miR-375, are altered in response to a glucose challenge.

Design: Twelve healthy males were recruited for an acute cross-over study which consisted of two tests each following an eight-hour fasting period. An oral glucose tolerance test (OGTT) was performed and blood samples were collected over a 3-hour period. Following a period of at least one week, the same participants were administered an isoglycemic intravenous glucose infusion (IIGI) with the same blood collection protocol.

Results: The glucose response curve following the IIGI mimicked that obtained after the OGTT, but as expected systemic insulin levels were lower during the IIGI compared to the OGTT (P<0.05). MiR-375 levels in circulation were increased only in response to an OGTT and not during an IIGI. In addition, the response to the OGTT also coincided with the transient increase of circulating glucagon-like peptide-1 (GLP-1), glucagon-like peptide-2 (GLP-2), and glucose-dependent insulinotropic polypeptide (GIP).

Conclusions: The present findings show levels of miR-375 increase following administration of an OGTT and in light of its enrichment in cells of the gut, suggest that the gastrointestinal tract may play a significant role to the abundance and function of this microRNA in the blood.

OriginalsprogEngelsk
TidsskriftJournal of Clinical Endocrinology and Metabolism
Vol/bind102
Tidsskriftsnummer10
Sider (fra-til)3749-3755
Antal sider7
ISSN0021-972X
DOI
StatusUdgivet - 1 okt. 2017

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