The urinary metabolome in female mink (Mustela neovison) shows distinct changes in protein and lipid metabolism during the transition from diapause to implantation

Publikation: Forskning - peer reviewTidsskriftartikel

DOI

Introduction
The mink exhibit an obligatory diapause. The metabolic changes during the transition from diapause to implantation and established pregnancy are currently unknown.

Objectives
The study aimed to characterize changes in the urinary metabolome in mink during the period from mating to early gestation and to identify the metabolites involved.

Methods
Urine samples were collected from 56 female mink on March 24, April 8, and April 15, covering the period from mating to early pregnancy. The urine samples were subjected to non-targeted LC-MS metabolomics. Processed data were evaluated by principal component analysis (PCA) and the peak area of identified metabolites were subjected to ANOVA.

Results
The samples showed clear clustering according to sampling date in a PCA scores plot, and 35 metabolites differing significantly between sampling days were identified. The excretion of dicarboxylic acids and acylcarnitines of dicarboxylic acids exhibited a decline on April 8, and the same trend was observed for four unidentified metabolites, two of which were putatively identified as acids of the furan fatty acid type. The decreased excretion of lipid components was suggested to be a result of increased oxidation of these compounds. In contrast, the excretion of amino acid-related metabolites showed an increase on April 8 which was attributed to increased metabolism of amino acids at this time point.

Conclusion
The urinary metabolic profile of mink showed distinct changes during the period studied. The major changes were observed at the time of implantation where increases in the lipid and protein metabolism were evident.
OriginalsprogEngelsk
Artikelnummer64
TidsskriftMetabolomics
Vol/bind13
Tidsskriftsnummer5
Antal sider13
ISSN1573-3882
DOI
StatusUdgivet - maj 2017

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