Giuseppe Dionisio

Akademisk medarbejder

Giuseppe Dionisio
Se relationer på Aarhus Universitet

Profil

The topics of my research include Plant Molecular Biology, Proteomics, Physiology and Biochemistry.  Enzymology of plant proteins. In relation to plant biotechnology my past achievements have been based on grain hydrolases characterization as biochemically purified from the plant sources or recombinant produced in Escherichia coli or yeast. Plant transformation with such hydrolases (Transgenic or Cis-genic approach) have validated their role in a specific biochemical pathway (i.e. purple acid phosphatases with phytase activity in phytate degradation). Other plant enzymes of interest are cereal grains hydrolases in particular different classes of proteases (aspartic, serine, cysteine types, etc.) and proteases inhibitors (i.e. chymotrypsin inhibitors, serpins, Bowmann-Birk, etc.). Their biochemical characterization, post translational modifications discovery by Mass Spectroscopy including absolute differential proteomics is currently performed under my supervision using enzymes extracted from the grains or recombinant enzymes overexpressed either in planta or in heterologous hosts (E. coli, Pichia pastoris, etc.). One of the topic in which I am currently working on is proteolytic feed enzymes from plant/fungi and their interaction with endogenous cereal proteinaceous inhibitors.  Another topic I am interested and working also is the Biopharming of Human pathogen virus as Virus Like Particles (VLPs) for candidate vaccine development in cereal grains (i.e. Hepatitis B surface antigen). Furthermore, one of my last topic of my research is metabolic engineering of carrot transcription factors (MYB/bHLH) involved in purple color of taproots by using overexpression techniques like cis-genesis and new knock-out methods like CRISPR/Cas9. The range of my collaboration includes Novozymes, Carlsberg, Chr. Hansen, Sejet Plant Breeding, Copenhagen University, University of East Anglia, Norwich UK, Leibniz Institut, Freising, Germany and others). New topics where I am specializing are: A) Bio-insecticides production based on recombinant entomopathogenic fungal enzymes and their in planta transgenic expression. B) Protease inhibitors biosynthesis by NonRibosomal Peptide Synthetases (NRPS) and recombinant based. C) Development of immunological based kits for plant pathogen detection.

ID: 6432